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JSIR Vol.68 [2009] >
JSIR Vol.68(08) [August 2009] >
| Title: | Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases |
| Authors: | Das, Arpan
Ghosh, Uma |
| Keywords: | CMC
FPA
SSF
Mixed substrate
Penicillium sp. |
| Issue Date: | Aug-2009 |
| Publisher: | CSIR |
| Abstract: | Solid-state fermentation (SSF) of waste cabbage was carried out by Penicillium notatum NCIM NO-923 for production of extracellular enzymes. Highest activities of carboxymethyl cellulase (CMC) (817.19 ±1.2 IU/gds) was obtained at 30°C and 48h fermentation time and that of filter paper activity (FPA) (67.49 ±1.33IU/gds) was obtained at 30°C and 24 h fermentation time. SSF of mixed substrate (cabbage and bagasse; 3:2 w/w) resulted in increase in CMC activity by 3277.3 ±1.22 and FPAby1280.76 ±1.33. Kinetic parameters were determined from Linewaver Burk plot. Both enzymes exhibited significant thermo stability up to 50°C. |
| Page(s): | 714-718 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.68(08) [August 2009]
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