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IJEB Vol.45(04) [April 2007] >


Title: Evaluation of free radical scavenging activity of Butea monosperma Lam.
Authors: Lavhale, Manish S
Mishra, S H
Keywords: Butea monosperma
Free radicals
HPTLC fingerprint
Radical scavenging
Issue Date: Apr-2007
Publisher: CSIR
Abstract: In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74%w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts.
Page(s): 376-384
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(04) [April 2007]

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