Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5262
Title: Impediment of diethylnitrosamine induced hepatotoxicity in male Balb/c mice by pretreatment with aqueous <i style="">Azadirachta indica </i>leaf extract
Authors: Koul, Ashwani
Binepal, Gursonika
Gangar, Subhash Chander
Keywords: <i style="">Azadirachta indica</i>
Biotransformation
Diethylnitrosamine
Lactate dehydrogenase
Micronucleus
Oxidative stress
Issue Date: Apr-2007
Publisher: CSIR
Abstract: Considering the hepatoprotective properties of <i style="">Azadirachta indica, </i>the present study was designed to evaluate its preventive effects against diethylnitrosamine (NDEA) induced hepatotoxicity in male Balb/c mice. Exposure of NDEA caused a significant increase in micronucleated cell score, lipid peroxidation levels (LPO) and activity of lactate dehydrogenase (LDH). A significant decrease in reduced glutathione (GSH) contents and activity of glutathione-S-transferase (GST) was also observed upon NDEA treatment, whereas their activities of cytochrome P450 and cytochrome b<sub>5</sub> showed non-significant alterations. Aqueous <i style="">A. indica </i>leaf extract (AAILE) pretreatment showed protective effects against NDEA induced toxicity by decreasing the frequency of micronucleated cell, levels of LPO and LDH activity. Also, a decreased activity of GST, cytochrome P450 and an increased activity of cytochrome b<sub>5</sub>, GSH contents was observed when AAILE pretreated mice were injected with NDEA. Only AAILE treatment caused a noticeable decrease in the frequency of micronuclei, activity of cytochrome P450 and cytochrome b<sub>5</sub>, but a significant increase in the activity of GST and GSH contents, whereas, non significant alterations were observed in the activity of LDH and levels of LPO. Significance of these observations with respect to hepatoprotective efficacy of <i style="">A. indica </i>has been discussed in the present manuscript.
Description: 359-366
URI: http://hdl.handle.net/123456789/5262
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.45(04) [April 2007]

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