Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5249
Title: Characterization of antimicrobial compounds from a common fern, <i style="">Pteris biaurita</i>
Authors: Dalli, A K
Saha, G
Chakraborty, U
Keywords: Antimicrobial compounds
Fern
HPLC
GC-MS
Issue Date: Mar-2007
Publisher: CSIR
Abstract: Methanol extract was prepared from the fronds of <i style="">Pteris biaurita</i> and partial purification was done by solvent partitioning with diethyl ether and ethyl acetate, followed by hydrolysis and further partitioning with ethyl acetate. The three fractions, thus obtained were bioassayed separately against five test fungi- <i style="">Curvularia lunata</i>, <i style="">Fomes lamaoensis</i>, <i style="">Poria hypobrumea</i>, <i style="">Fuasrium oxysporum</i> and a bacterium- <i style="">Bacillus pumilus</i>, by spore germination, radial growth and agar cup techniques. Results revealed that ethyl acetate fraction (III) contained the active principle. TLC plate bioassay of the active fraction revealed inhibition zone at an R<sub>f </sub>of 0.5-0.65. Silica gel from this region was scraped, eluted in methanol and subjected to UV-spectrophotometric analysis. An absorption maxima of 278 nm was recorded. HPLC analysis of TLC-eluate revealed a single peak with retention time of 8.1 min. GC-MS analysis revealed six major peaks in the retention time range of 7.2-10.9 min. Comparison with GC-MS libraries revealed that the extracts may contain a mixture of eicosenes and heptadecanes.
Description: 285-290
URI: http://hdl.handle.net/123456789/5249
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.45(03) [March 2007]

Files in This Item:
File Description SizeFormat 
IJEB 45(3) 285-290.pdf544.76 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.