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IJEB Vol.45(03) [March 2007] >

Title: Liposomes as adjuvant for combination vaccines
Authors: Mishra, Neeraj
Gupta, Prem N
Mahor, Sunil
Khatri, Kapil
Goyal, Amit K
Vyas, Suresh P
Keywords: Adjuvants
Combination vaccines
Diphtheria toxoid
Tetanus toxoid
Issue Date: Mar-2007
Publisher: CSIR
Abstract: In the present study tetanus toxoid (TT) loaded liposomes and diphtheria toxoid (DT) loaded liposomes were prepared by reverse phase evaporation method and after combining these two vaccines the potential advantages were investigated. Prepared systems were characterized for the size, shape and entrapment efficiency. SDS-PAGE analysis of TT and DT was also performed. The selected liposomal formulations were administered subcutaneously to Balb/c mice and their immune responses were determined using ELISA after 15, 30, 45 days. After boosting the maximum immune response was observed after 45 days and was found to be 0.831 and 0.749 for TT loaded liposome and DT loaded liposomes respectively. When the mice were immunized subcutaneously with the physical mixture of TT loaded liposomes and DT loaded liposomes the immune response for the combination vaccine was found to be 1.44 and 0.741 for the TT and DT respectively. The result showed that the immune response of TT increased when it was combined with DT in liposomes. This confirms adjuvantcity of DT vis-a-vis immunogenicity. Thus, carrier mediated cocktail vaccination holds promise for clinical applications.
Page(s): 237-241
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.45(03) [March 2007]

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