|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.45 [2007] >
IJEB Vol.45(02) [February 2007] >
| Title: | Verotoxic Escherichia coli (STEC) from beef and its products |
| Authors: | Hazarika, R A
Singh, D K
Kapoor, K N
Agarwal, R K
Pandey, A B
Purusottam |
| Keywords: | Animal products
Beef
Escherichia coli
STEC
Verotoxin |
| Issue Date: | Feb-2007 |
| Publisher: | CSIR |
| Abstract: | In the present investigation, out of 27 (24.10%) strains of Escherichia coli isolated from 112 beef samples comprising raw meat (45), kabab (36) and kofta (31), 9 (33.33%) belonging to 7 different serotypes were verotoxic as tested by vero cell cytotoxic assay. Serotype O145 was the predominant STEC in raw meat. Interestingly, one STEC-O157 strain was also detected. All the STEC strains were positive for Stx genes by polymerase chain reaction showing stx2 (77.78%) to be most predominant followed by stx1 (22.22%). Phenotypic enterohaemolysin production on washed sheep blood agar supplemented with CaCl2 revealed 6 (66.67%) STEC strains to be positive. Presence of STEC in cooked beef products, viz., kabab and kofta appeared to be a matter of concern and potential threat to public health. |
| Page(s): | 207-211 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.45(02) [February 2007]
|
|
