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Title: Ultraviolet-C haematogenous oxidation therapy of lipopolysaccharide-induced endotoxemia in a rabbit model: A biochemical study
Authors: Adam, Gareeballah Osman
Kim, Gi-Beum
Lee, Sei-Jin
Lee, Heeryung
Kim, Shang-Jin
Kim, Jin-Shang
Kang, Hyung-Sub
Keywords: Lactate;Lipopolysaccharide (LPS);Magnesium;Metabolic acidosis;Sepsis;Rabbits;Ultraviolet Haematogenous Oxidation Therapy (UV-C HOT)
Issue Date: Dec-2019
Publisher: NISCAIR-CSIR, India
Abstract: Systemic inflammatory reaction – due to severe response to toxins of infection associated with immune inhibition – leads to multi-organ dysfunctions and high mortality. Ultraviolet (UV) blood is used for its therapeutic effects when moving across the cells. This study aims to evaluate the impact of UV-C Haematogenous Oxidation Therapy (HOT) in Lipopolysaccharide (LPS)-induced endotoxemia of rabbit model. A total of 40 rabbits randomly divided into four groups, including normal control (NC). LPS and LPS+UV-C HOT groups received 0.1 mg/kg LPS toxin of E. coli, UV-C HOT and LPS+UV-C HOT groups subjected to UV-C HOT treatments once weekly for five times. Blood collected, perfused with oxygen, UV-C directly irradiated into blood, and then auto-transfused. Rabbits were sacrificed after five weeks; blood and serum were collected for analysis. The survival rate, liver, kidney, lipid profile, and blood ions were assessed in treated rabbits. Mortality was 40% in the LPS group, while other groups showed no death. UV-C HOT enhanced critical pH, base deficit, blood gases, hypomagnesemia, hyperlactatemia, and concurrent acidosis. Besides, TNF-α, nitrite, and nitrate were suppressed in response to UV-C HOT. Moreover, UV-C HOT reduced liver and kidney enzymes, improved lipid metabolism, and ameliorated electrolytes homeostasis. Despite that, UV-C HOT performance in ICU for human and animal endotoxemic or septic patients should be evaluated and considered.
Page(s): 445-454
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.56(6) [December 2019]

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