Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5141
Title: Purification of phycobiliproteins from Nostoc muscorum
Authors: Ranjitha, K
Kaushik, B D
Keywords: Nostoc muscorum
Phycobiliproteins
Phycoerythrin
Phycocyanin
Allophycocyanin
Issue Date: May-2005
Publisher: CSIR
Series/Report no.: C 12 N 1/12
Abstract: Purification of phycobiliproteins from phycoerythrin (PE) rich strain of Nostoc muscorum was carried out. Salting out with 55 % (NH4)2SO4 precipitates >80 % of each class of phycobiliprotein. Chromatography on DEAE cellulose-52 column gives pure PE (A562/A280 = 8.12), while rechromatography of the blue coloured fraction from this column using a hydroxy apatite column yields pure phycocyanin (PC) (A615/A280 = 3.89). SDS-PAGE analysis of purified PE and PC yields two polypeptides (~19.4 and 16.9 kDa). PE is found to be stable between pH 5-9. This protocol helps to get highly pure PE (72%) and PC (39%).
Description: 372-375
URI: http://hdl.handle.net/123456789/5141
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.64(05) [May 2005]

Files in This Item:
File Description SizeFormat 
JSIR 64(5) 372-375.pdf209.47 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.