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|Title:||Purification of phycobiliproteins from Nostoc muscorum|
Kaushik, B D
|Series/Report no.:||C 12 N 1/12|
|Abstract:||Purification of phycobiliproteins from phycoerythrin (PE) rich strain of Nostoc muscorum was carried out. Salting out with 55 % (NH4)2SO4 precipitates >80 % of each class of phycobiliprotein. Chromatography on DEAE cellulose-52 column gives pure PE (A562/A280 = 8.12), while rechromatography of the blue coloured fraction from this column using a hydroxy apatite column yields pure phycocyanin (PC) (A615/A280 = 3.89). SDS-PAGE analysis of purified PE and PC yields two polypeptides (~19.4 and 16.9 kDa). PE is found to be stable between pH 5-9. This protocol helps to get highly pure PE (72%) and PC (39%).|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.64(05) [May 2005]|
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