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Title: Microsatellite markers for genetic characterisation of Kachchhi camel
Authors: Mehta, S C
Goyal, A
Sahani, M S
Keywords: breeds;camel;genetic characterisation;Kachchhi;microsatellite
Issue Date: Jul-2007
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/10, 15/16
Abstract: Sixteen microsatellite loci were investigated for studying the genetic polymorphism in Kachchhi breed of camel. Polymerase chain reactions were carried out for 50 unrelated camels of Kachchhi breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 16 microsatellite loci, 13 were found polymorphic. The number of alleles ranged from 2 to 6. The expected heterozygosity ranged from 0.332 to 0.796. The polymorphic information content ranged from 0.277 to 0.765. The results indicated existence of enough genetic variation among dromedary individuals and the potential use of microsatellite markers for further genetic investigations including genetic diversity analysis, individual identification, parentage testing and production enhancement.
Page(s): 336-339
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(3) [July 2007]

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