NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.64 [2005] >
JSIR Vol.64(07) [July 2005] >


Title: Production, optimization and characterization of extracellular invertase by an actinomycete strain
Authors: Kaur, N
Sharma, A D
Keywords: Fructose syrups
Invertase
Streptomyces sp.
Issue Date: Jul-2005
Publisher: CSIR
Abstract: Streptomyces sp. ALKC8 produced high levels of extra-cellular invertase (350 Ul-1) after incubation for 24 h at pH 5.0, temperature 37°C in the presence of sucrose (3%). The optimum temperature and pH for enzyme activity were 60°C and 5.0 respectively. All metal ions except Na+, Hg2+ and Ca2+ were well tolerated and did not adversely affect invertase activity. However, SDS inhibited the enzyme activity. The effect of thermal stabilizers on invertase activity was studied. Consequently, polyethylene glycol and mannitol were shown to have a protective effect on enzyme activity. Maximum invertase activity (581 Ul-1) was produced by cells grown in the medium containing sucrose (2% w/v) and yeast extract in combination with NaNO3, which was about twofold higher than the production in basal medium.
Page(s): 515-519
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.64(07) [July 2005]

Files in This Item:

File Description SizeFormat
JSIR 64(7) 515-519.pdf135.84 kBAdobe PDFView/Open
 Current Page Visits: 655 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 576465 since 06-Feb-2009  Last updated on 26-Aug-2014Webmaster: nopr@niscair.res.in