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Title: Plant regeneration through somatic embryogenesis from stem and petiole explants of Indian chicory (Cichorium intybus L.)
Authors: Abdin, M Z
Ilah, A
Keywords: Cichorium intybus L.;Indian chicory;micropropagation;somatic embryogenesis
Issue Date: Apr-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00, 5/10
Abstract: An efficient protocol has been developed for rapid propagation of Indian chicory (Cichorium intybus L.) through somatic embryo development. Indirect somatic embryogenesis was induced through nodal stem and petiole explants, cultured on Murashige and Skoog (MS) medium containing different concentrations of 2,4-D, NAA or IAA. Callus was induced from both the explants after 3 wk of inoculation. Subsequently, calli were sub-cultured on MS medium containing different auxin/cytokinin ratios that influenced the intensity of embryo formation, germination and ability to regenerate plants. Somatic embryogenesis was more intensive in medium with higher concentration of Kn (1.5 mg L-1) and lower concentration of IAA (0.5 mg L-1) supplemented with vitamin-free casein hydrolysate (500 mg L-1). After sub-culture, the embryoids were matured onto a fresh MS medium supplemented with Kn (1.5 mg L-1) + IAA (0.1 mg L-1) + IBA (1.0 mg L-1) + CH (500 mg L-1). The germination of embryos into shoots and less developed roots occurred on the same medium with slight change in IBB concentration (0.5 mg L-1). Further, root formation occurred in MS medium supplemented with 1.0 mg L-1 IBA.
Page(s): 250-255
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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