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Title: Plant regeneration through somatic embryogenesis from stem and petiole explants of Indian chicory (<i style="">Cichorium intybus</i> L.)
Authors: Abdin, M Z
Ilah, A
Keywords: <i style="">Cichorium intybus</i> L.
Indian chicory
somatic embryogenesis
Issue Date: Apr-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00, 5/10
Abstract: An efficient protocol has been developed for rapid propagation of Indian chicory (<i style="">Cichorium intybus L.</i>) through somatic embryo development. Indirect somatic embryogenesis was induced through nodal stem and petiole explants, cultured on Murashige and Skoog (MS) medium containing different concentrations of 2,4-D, NAA or IAA. Callus was induced from both the explants after 3 wk of inoculation. Subsequently, calli were sub-cultured on MS medium containing different auxin/cytokinin ratios that influenced the intensity of embryo formation, germination and ability to regenerate plants. Somatic embryogenesis was more intensive in medium with higher concentration of Kn (1.5 mg L<sup>-1</sup>) and lower concentration of IAA (0.5 mg L<sup>-1</sup>) supplemented with vitamin-free casein hydrolysate (500 mg L<sup>-1</sup>). After sub-culture, the embryoids were matured onto a fresh MS medium supplemented with Kn (1.5 mg L<sup>-1</sup>) + IAA (0.1 mg L<sup>-1</sup>) + IBA (1.0 mg L<sup>-1</sup>) + CH (500 mg L<sup>-1</sup>). The germination of embryos into shoots and less developed roots occurred on the same medium with slight change in IBB concentration (0.5 mg L<sup>-1</sup>). Further, root formation occurred in MS medium supplemented with 1.0 mg L<sup>-1 </sup>IBA.
Description: 250-255
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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