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Title: Bioactive Compounds characterization and Antibacterial Potentials of Actinomycetes isolated from Rhizospheric soil
Authors: Kumari, Nalinee
Menghani, Ekta
Mithal, Rekha
Keywords: Secondary metabolites;Rhizosphere;Actinomycetes;Antimicrobial Activity;Antibiotics
Issue Date: Nov-2019
Publisher: NISCAIR-CSIR, India
Abstract: Due to acquired resistance produced by micro-organism against all frequently used antimicrobial agents, there is a great need of newer antibiotics to fight against different microbes. To explore the unknown diversity of microorganisms, soil screening for antimicrobial agents are performed for the detection of newer antibiotics from Actinomycetes. Studies were carried out with isolation of total 65 actinomycetes from rhizospheric soil of plants. Isolation and characterization of antimicrobial agents producing actinomycetes was performed and after screening some of isolates were having antimicrobial activity. Among them isolate AIA29 from rhizospheric soil was further studied for their potential. In the present study soil samples have been collected from different regions of Rajasthan. Isolation of actinomycetes was done on Actinomycetes Isolation Agar (AIA) media. Gram staining, characterization of colony and extraction of bioactive compounds was done by solvent extraction method. Antibacterial screening of crude extract was also performed against selected organisms like Staphylococcus aureus (MTCC-3160), Pseudomonas aeruginosa (MTCC-1688), Klebsiella pneumonia (MTCC-432), Proteus vulgaris (MTCC-7306), Bacillus subtilis (MTCC-441) procured from IMTECH Chandigarh. The extract showed inhibition zones (IZ) against Staphylococcus aureus (IZ= 33mm), Proteus vulgaris (IZ= 23mm) and Bacillus subtilis (IZ= 28mm). In GCMS analysis various compounds have been identified and compounds like Skatole, 3-methylpentane, Cyclohexane, Hexadecane, linoleic acid, Heptadecane, Nonadecane, Cyclotetradecane, Triacontane, Vinylbital, Ethosuximide and Stearyl alcohol, Dioctylpthalate was identified as having various activity.
Page(s): 793-798
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.78(11) [November 2019]

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