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|Title:||Production and partial characterization of alkali-tolerant xylanase from an alkalophilic <i style="">Streptomyces</i> sp. CD3|
Bajaj, Bijender K
|Abstract:||Of the 30 isolates screened, <i style="">Streptomyces</i> sp CD3, an alkalophilic isolate, showed maximum xylanase production. Organism efficiently used wheat bran and bagasse as substrates and produced 2.211 and 1.896 U/ml of xylanase, respectively. Soybean meal supplementation enhanced xylanase production substantially, while yeast extract and gelatin did so moderately. Although, optimum enzyme activity was at pH 8 and temperature (50<sup>o</sup>C), but enzyme retained considerable activity at higher pH (80% at 9-10) and temperature (60 % at 70-90<sup>o</sup>C). The enzyme was strongly inhibited by Hg<sup>2+</sup>, while Fe<sup>3+</sup>, Ca<sup>2+</sup> and Zn<sup>2+</sup> were slight inducers of xylanase. Zymogram analysis suggested the presence of three xylanases (mol wt, 69.18, 63.09 & 43.65 kDa). Purification (108-fold) was achieved by carboxymethyl sephadex chromatography. Enzyme obeyed Michaelis-Menten kinetics (K<sub>m</sub> 3.9 mg/ml). Industrially desirable characteristics of the enzyme like thermostability and alkali-stability, and highly alkalophilic nature of the organism, and its ability to grow and produce enzyme on low value agricultural by-products reflects the potential commercial importance of this study.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.64(09) [September 2005]|
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