Please use this identifier to cite or link to this item:
Title: Production and partial characterization of alkali-tolerant xylanase from an alkalophilic Streptomyces sp. CD3
Authors: Sharma, Pankaj
Bajaj, Bijender K
Keywords: Streptomyces;Xylanase;Lignocellulosics;Thermostable;Alkalophilic;Alkali-tolerant;Zymogram
Issue Date: Sep-2005
Publisher: CSIR
IPC Code: A61K38/47
Abstract: Of the 30 isolates screened, Streptomyces sp CD3, an alkalophilic isolate, showed maximum xylanase production. Organism efficiently used wheat bran and bagasse as substrates and produced 2.211 and 1.896 U/ml of xylanase, respectively. Soybean meal supplementation enhanced xylanase production substantially, while yeast extract and gelatin did so moderately. Although, optimum enzyme activity was at pH 8 and temperature (50oC), but enzyme retained considerable activity at higher pH (80% at 9-10) and temperature (60 % at 70-90oC). The enzyme was strongly inhibited by Hg2+, while Fe3+, Ca2+ and Zn2+ were slight inducers of xylanase. Zymogram analysis suggested the presence of three xylanases (mol wt, 69.18, 63.09 & 43.65 kDa). Purification (108-fold) was achieved by carboxymethyl sephadex chromatography. Enzyme obeyed Michaelis-Menten kinetics (Km 3.9 mg/ml). Industrially desirable characteristics of the enzyme like thermostability and alkali-stability, and highly alkalophilic nature of the organism, and its ability to grow and produce enzyme on low value agricultural by-products reflects the potential commercial importance of this study.
Page(s): 688-697
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.64(09) [September 2005]

Files in This Item:
File Description SizeFormat 
JSIR 64(9) 688-697.pdf487.84 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.