NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.64 [2005] >
JSIR Vol.64(09) [September 2005] >


Title: Production and partial characterization of alkali-tolerant xylanase from an alkalophilic Streptomyces sp. CD3
Authors: Sharma, Pankaj
Bajaj, Bijender K
Keywords: Streptomyces
Xylanase
Lignocellulosics
Thermostable
Alkalophilic
Alkali-tolerant
Zymogram
Issue Date: Sep-2005
Publisher: CSIR
IPC CodeA61K38/47
Abstract: Of the 30 isolates screened, Streptomyces sp CD3, an alkalophilic isolate, showed maximum xylanase production. Organism efficiently used wheat bran and bagasse as substrates and produced 2.211 and 1.896 U/ml of xylanase, respectively. Soybean meal supplementation enhanced xylanase production substantially, while yeast extract and gelatin did so moderately. Although, optimum enzyme activity was at pH 8 and temperature (50oC), but enzyme retained considerable activity at higher pH (80% at 9-10) and temperature (60 % at 70-90oC). The enzyme was strongly inhibited by Hg2+, while Fe3+, Ca2+ and Zn2+ were slight inducers of xylanase. Zymogram analysis suggested the presence of three xylanases (mol wt, 69.18, 63.09 & 43.65 kDa). Purification (108-fold) was achieved by carboxymethyl sephadex chromatography. Enzyme obeyed Michaelis-Menten kinetics (Km 3.9 mg/ml). Industrially desirable characteristics of the enzyme like thermostability and alkali-stability, and highly alkalophilic nature of the organism, and its ability to grow and produce enzyme on low value agricultural by-products reflects the potential commercial importance of this study.
Page(s): 688-697
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.64(09) [September 2005]

Files in This Item:

File Description SizeFormat
JSIR 64(9) 688-697.pdf487.84 kBAdobe PDFView/Open
 Current Page Visits: 631 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 569645 since 06-Feb-2009  Last updated on 14-Aug-2014Webmaster: nopr@niscair.res.in