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JSIR Vol.64 [2005] >
JSIR Vol.64(09) [September 2005] >
| Title: | Production and partial characterization of alkali-tolerant xylanase from an alkalophilic Streptomyces sp. CD3 |
| Authors: | Sharma, Pankaj
Bajaj, Bijender K |
| Keywords: | Streptomyces
Xylanase
Lignocellulosics
Thermostable
Alkalophilic
Alkali-tolerant
Zymogram |
| Issue Date: | Sep-2005 |
| Publisher: | CSIR |
| IPC Code: | A61K38/47 |
| Abstract: | Of the 30 isolates screened, Streptomyces sp CD3, an alkalophilic isolate, showed maximum xylanase production. Organism efficiently used wheat bran and bagasse as substrates and produced 2.211 and 1.896 U/ml of xylanase, respectively. Soybean meal supplementation enhanced xylanase production substantially, while yeast extract and gelatin did so moderately. Although, optimum enzyme activity was at pH 8 and temperature (50oC), but enzyme retained considerable activity at higher pH (80% at 9-10) and temperature (60 % at 70-90oC). The enzyme was strongly inhibited by Hg2+, while Fe3+, Ca2+ and Zn2+ were slight inducers of xylanase. Zymogram analysis suggested the presence of three xylanases (mol wt, 69.18, 63.09 & 43.65 kDa). Purification (108-fold) was achieved by carboxymethyl sephadex chromatography. Enzyme obeyed Michaelis-Menten kinetics (Km 3.9 mg/ml). Industrially desirable characteristics of the enzyme like thermostability and alkali-stability, and highly alkalophilic nature of the organism, and its ability to grow and produce enzyme on low value agricultural by-products reflects the potential commercial importance of this study. |
| Page(s): | 688-697 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.64(09) [September 2005]
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