NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >


Title: Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae)
Authors: Shadang, R
Dwivedi, Padmanabh
Hegde, S N
Ahmed, N
Keywords: culture media
Hygrochilus parishii
orchid
protocorms
seed germination
Issue Date: Apr-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: Seeds of Hygrochilus parishii (Veith & Rchb.f.) Pfitz were inoculated on eight different culture media (basal) MS, MKC, V&W, I&Y and half strength of respective media. Seeds germinated within 15-20 d almost on all media with varying percentage and developed into greenish protocorm after 80-100 d. Best seed germination was observed in ½ strength MKC medium. Healthy protocorms were observed within 100 d in V&W media. Sub-cultured protocorms in all the above media supplemented with NAA, BAP, 2,4-D and IAA in a range of 0.5-2.0 mg L-1, both individually and in combination, and additives like banana pulp (BP, 10%) and coconut milk (CM, 15%) produced well developed callus. Multiplication of protocorms was best on ½ strength MS + NAA (2.0 mg L-1). Formation of root and leaf was early in V&W medium having CM (15%) and BP (10%) but without sucrose.
Page(s): 256-261
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source: IJBT Vol.06(2) [April 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(2) 256-261.pdf315.9 kBAdobe PDFView/Open
 Current Page Visits: 674 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 603060 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in