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Title: Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae)
Authors: Shadang, R
Dwivedi, Padmanabh
Hegde, S N
Ahmed, N
Keywords: culture media;Hygrochilus parishii;orchid;protocorms;seed germination
Issue Date: Apr-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00
Abstract: Seeds of Hygrochilus parishii (Veith & Rchb.f.) Pfitz were inoculated on eight different culture media (basal) MS, MKC, V&W, I&Y and half strength of respective media. Seeds germinated within 15-20 d almost on all media with varying percentage and developed into greenish protocorm after 80-100 d. Best seed germination was observed in ½ strength MKC medium. Healthy protocorms were observed within 100 d in V&W media. Sub-cultured protocorms in all the above media supplemented with NAA, BAP, 2,4-D and IAA in a range of 0.5-2.0 mg L-1, both individually and in combination, and additives like banana pulp (BP, 10%) and coconut milk (CM, 15%) produced well developed callus. Multiplication of protocorms was best on ½ strength MS + NAA (2.0 mg L-1). Formation of root and leaf was early in V&W medium having CM (15%) and BP (10%) but without sucrose.
Page(s): 256-261
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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