|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >
| Title: | Effects of different culture media on seed germination and subsequent in vitro development of protocorms of Hygrochilus parishii (Veith & Rchb.f.) Pfitz (Orchidaceae) |
| Authors: | Shadang, R
Dwivedi, Padmanabh
Hegde, S N
Ahmed, N |
| Keywords: | culture media
Hygrochilus parishii
orchid
protocorms
seed germination |
| Issue Date: | Apr-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | Seeds of Hygrochilus parishii (Veith & Rchb.f.) Pfitz were inoculated on eight different culture media (basal) MS, MKC, V&W, I&Y and half strength of respective media. Seeds germinated within 15-20 d almost on all media with varying percentage and developed into greenish protocorm after 80-100 d. Best seed germination was observed in ½ strength MKC medium. Healthy protocorms were observed within 100 d in V&W media. Sub-cultured protocorms in all the above media supplemented with NAA, BAP, 2,4-D and IAA in a range of 0.5-2.0 mg L-1, both individually and in combination, and additives like banana pulp (BP, 10%) and coconut milk (CM, 15%) produced well developed callus. Multiplication of protocorms was best on ½ strength MS + NAA (2.0 mg L-1). Formation of root and leaf was early in V&W medium having CM (15%) and BP (10%) but without sucrose. |
| Page(s): | 256-261 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(2) [April 2007]
|
|
