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Title: Standardization of Centipeda minima, Mimusops elengi, Orchis mascula and Paeonia emodii
Authors: Singh, Rajbir
Kachroo, Monika
Agrawal, Shyam S
Keywords: Chromatography
Indian Systems of Medicine
Issue Date: Jul-2009
Publisher: CSIR
IPC CodeInt. Cl.8: A61K36/00, A61P3/04, A61P3/06
Abstract: TLC/HPTLC profiles with other physiochemical parameters are good tools for standardization and validation of medicinal plants, and are simple and effective methods for determination of the phytochemicals. The study developed TLC/HPTLC methods (optimal solvent system) for phytochemical analysis of five medicinal plants used in Indian Systems of Medicine (whole dried plant of Centipeda minima; dried flowers and fruits of Mimusops elengi; dried root tubers of Orchis mascula and dried root tubers of Paeonia emodii). TLC and HPTLC of ethanolic extracts of C. minima whole herb, M. elengi flower, M. elengi fruit, methanolic extracts of O. mascula root tuber and P. emodi root tuber were compared using several solvent systems including one mentioned in ISM report. Results of both TLC and HPTLC analysis suggests benzene: ethyl acetate (8:2) solvent system for C. minima; benzene: ethyl acetate: glacial acetic acid (6:3:1) for M. elengi flower; benzene: ethyl acetate (8:2) for M. elengi fruits; ethanol: chloroform (7:3) and chloroform: methanol: glacial acetic acid (6:3:1) for O. mascula root tuber and ethanol: chloroform (5:5) solvent system for P. emodi root tuber.
Page(s): 338-345
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Source:IJTK Vol.08(3) [July 2009]

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