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Title: Physical mapping of rRNA gene in endangered fish <i style="">Osteobrama belangeri</i> (Valenciennes, 1844) (Family: Cyprinidae)
Authors: Kumar, Ravindra
Kushwaha, B
Nagpure, N S
Behera, B K
Srivastava, S K
Lakra, W S
Keywords: Ag-NORs
<i style="">Osteobrama belangeri</i>
Issue Date: Jul-2009
Publisher: CSIR
Abstract: Physical mapping of the 18S ribosomal RNA gene (rDNA) was carried out by fluorescent <i style="">in situ</i> hybridization (FISH) in the endangered freshwater fish <i style="">O. belangeri</i>. The specimens were collected from Imphal valley, Manipur, India and metaphase chromosome preparation was made using standard hypotonic treatment, methanol-acetic acid fixation and flame-drying technique followed by Giemsa, silver, CMA<sub>3</sub> staining for complete cytogenetic characterization of the species. The diploid chromosome number was found to be 50 and the karyotype composed of 6m+16sm+12st+16t (FN=72). One active rDNA site, located on short arm of 3<sup>rd</sup> submetacentric chromosome, was mapped by FISH and confirmed by silver and CMA<sub>3</sub> staining. The karyomorphology, chromosomal location of rDNA loci in this species and the utility of these cytogenetic markers have been discussed in the paper.
Description: 597-601
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(07) [July 2009]

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