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Title: Mutation of <i style="">Alternaria tenuissima </i>FCBP-252 for hyper-active <img src='/image/spc_char/alpha.gif'> -amylase
Authors: Shafique, Sobiya
Bajwa, Rukhsana
Shafique, Shazia
Keywords: <i style="">Alternaria tenuissima</i>
EMS mutagenesis
UV mutagenesis
Issue Date: Jul-2009
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl=""> Production of extracellular α-amylase enzyme by a filamentous fungus, <i style="">Alternaria tenuissima</i> was studied in solid-state fermentation (SSF) as well as submerged fermentation (SmF). The potential strain was successfully mutated by UV and ethyl methanesulfonate (EMS). High-level of <img src='/image/spc_char/alpha.gif'> -amylase activity was obtained by the mutant At-Ch-5.6 (76.75 Units mL<sup>-1</sup>) after chemical treatment followed by UV mutant At-UV-2.8 (63.12 Units mL<sup>-1</sup>) which was significantly higher than parental <i style="">A. tenuissima </i>FCBP-252 (32 Units mL<sup>-1</sup>). These mutants with high levels of activity were genetically characterized using RAPD-PCR. Expression pattern of mutants exhibited that the mutants were isogenic variants of parent strain and out-performance of the mutants could be attributed to change in genetic make up. This work represented the first report of strain improvement in <i style="">Alternaria </i>for hyper activity of <img src='/image/spc_char/alpha.gif'> –amylase enzyme and suggested that this fungus could be used to extract purified enzyme. </smarttagtype>
Description: 591-596
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(07) [July 2009]

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