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Title: In vitro micropropagation of Heliotropium indicum Linn.—An Ayurvedic herb
Authors: Kumar, M Senthil
Rao, M V
Keywords: Heliotropium indicum
apical bud
axillary bud
Issue Date: Apr-2007
Publisher: CSIR
Series/Report no.: Int. Cl.8 A01H4/00
Abstract: An efficient micropropagation method was developed for Heliotropium indicum, an Ayurvedic medicinal herb, using apical and axillary bud explants. The highest number of shoots (32.6 shoots/apical bud and 20.2 shoots/axillary bud) was yielded after 30 d of culture in the MS medium supplemented with Kn (1.0 mg L-1), BA (0.5 mg L-1) and IAA (0.05 mg L-1). The cluster of proliferated shoots elongated simultaneously on the same medium. High frequency of rooting (85%) was obtained in both apical and axillary bud derived shoots when transferred to half-strength MS medium supplemented with IBA (0.1 mg L-1). Rooted plants were hardened and successfully established in field conditions.
Description: 245-249
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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