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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >
| Title: | In vitro micropropagation of Heliotropium indicum Linn.—An Ayurvedic herb |
| Authors: | Kumar, M Senthil
Rao, M V |
| Keywords: | Heliotropium indicum
Boraginaceae
micropropagation
apical bud
axillary bud |
| Issue Date: | Apr-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | An efficient micropropagation method was developed for Heliotropium indicum, an Ayurvedic medicinal herb, using apical and axillary bud explants. The highest number of shoots (32.6 shoots/apical bud and 20.2 shoots/axillary bud) was yielded after 30 d of culture in the MS medium supplemented with Kn (1.0 mg L-1), BA (0.5 mg L-1) and IAA (0.05 mg L-1). The cluster of proliferated shoots elongated simultaneously on the same medium. High frequency of rooting (85%) was obtained in both apical and axillary bud derived shoots when transferred to half-strength MS medium supplemented with IBA (0.1 mg L-1). Rooted plants were hardened and successfully established in field conditions. |
| Page(s): | 245-249 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(2) [April 2007]
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