Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5027
Title: <i style="">In vitro</i> micropropagation of <i style="">Heliotropium indicum</i> Linn.—An Ayurvedic herb
Authors: Kumar, M Senthil
Rao, M V
Keywords: <i style="">Heliotropium indicum</i>
<i style="">Boraginaceae</i>
micropropagation
apical bud
axillary bud
Issue Date: Apr-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00
Abstract: An efficient micropropagation method was developed for<i style=""> Heliotropium indicum</i>, an Ayurvedic medicinal herb, using apical and axillary bud explants. The highest number of shoots (32.6 shoots/apical bud and 20.2 shoots/axillary bud) was yielded after 30 d of culture in the MS medium supplemented with Kn (1.0 mg L<sup>-1</sup>), BA (0.5 mg L<sup>-1</sup>) and IAA (0.05 mg L<sup>-1</sup>). The cluster of proliferated shoots elongated simultaneously on the same medium. High frequency of rooting (85%) was obtained in both apical and axillary bud derived shoots when transferred to half-strength MS medium supplemented with IBA (0.1 mg L<sup>-1</sup>). Rooted plants were hardened and successfully established in field conditions.
Description: 245-249
URI: http://hdl.handle.net/123456789/5027
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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