NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >

Title: Micropropagation of mature tree of Citrus limon
Authors: Rathore, J S
Rathore, M S
Singh, M
Singh, R P
Shekhawat, N S
Keywords: cloning
ex vitro rooting
in vitro
mature explant
nodal shoot segment
Issue Date: Apr-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00
Abstract: A protocol for in vitro cloning of mature plants of Citrus limon using nodal shoot segments has been developed. Three to four shoots were induced per axillary meristems on MS medium amended with 9 µM BAP. Repeated transfer of the initial explants for up to six passages on MS medium [having 50% of the original concentrations of NH4NO3 and KNO3, and supplement of 250 mg L-1 (NH4)2SO4] with 0.22 µM BAP yielded crops of shoots. Three to four-fold shoot multiplication rate was achieved by subculturing of shoots. The shoots have been multiplied for more than 48 months without loss of vigour. One hundred percent of the cloned shoots rooted: (i) in vitro on half-strength MS medium+27 µM NAA+0.1% activated charcoal, and (ii) ex vitro on soilrite if pulse-treated with 1.07-2.68 mM NAA. Ninety five percent of rooted plantlets survived hardening and transplanting. Micropropagated plants are under filed trials in states of Rajasthan and Gujarat of India. These have flowered and produced fruits.
Page(s): 239-244
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source: IJBT Vol.06(2) [April 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(2) 239-244.pdf336.63 kBAdobe PDFView/Open
 Current Page Visits: 124 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171907 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: