Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5026
Title: Micropropagation of mature tree of <i style="">Citrus limon</i>
Authors: Rathore, J S
Rathore, M S
Singh, M
Singh, R P
Shekhawat, N S
Keywords: cloning
<i style="">ex vitro</i> rooting
<i style="">in vitro</i>
lemon
mature explant
nodal shoot segment
Issue Date: Apr-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00
Abstract: A protocol for <i style="">in vitro</i> cloning of mature plants of <i style="">Citrus limon</i> using nodal shoot segments has been developed. Three to four shoots were induced per axillary meristems on MS medium amended with 9 µ<i style="">M</i> BAP. Repeated transfer of the initial explants for up to six passages on MS medium [having 50% of the original concentrations of NH<sub>4</sub>NO<sub>3</sub> and KNO<sub>3,</sub> and supplement of 250 mg L<sup>-1</sup> (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>] with 0.22 µ<i style="">M</i> BAP yielded crops of shoots. Three to four-fold shoot multiplication rate was achieved by subculturing of shoots. The shoots have been multiplied for more than 48 months without loss of vigour. One hundred percent of the cloned shoots rooted: (i) <i style="">in vitro</i> on half-strength MS medium+27 µ<i style="">M</i> NAA+0.1% activated charcoal, and (ii) <i style="">ex vitro</i> on soilrite if pulse-treated with 1.07-2.68 m<i style="">M</i> NAA. Ninety five percent of rooted plantlets survived hardening and transplanting. Micropropagated plants are under filed trials in states of Rajasthan and Gujarat of India. These have flowered and produced fruits.
Description: 239-244
URI: http://hdl.handle.net/123456789/5026
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(2) [April 2007]

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