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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(1) [January 2007] >
| Title: | Standardization of in vitro protocol in Chrysanthemum cv. Madam E Roger for development of quality planting material and to induce genetic variability using  -radiation |
| Authors: | Misra, Pratibha
Datta, S K |
| Keywords: | Chrysanthemum
in vitro mutagenesis
quality planting material
shoot differentiation
-radiation
ray florets |
| Issue Date: | Jan-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00, 5/02; C12N15/01 |
| Abstract: | ‘Madam E Roger’, a greenish white large flowered chrysanthemum cultivar- was selected for in vitro propagation and mutagenesis to induce further genetic variability. A protocol has been standardized to develop large-scale quality planting material for commercial exploitation. Shoot bud differentiation could be achieved in ray florets in the presence of 0.5 mg L-1 NAA+ 2.0 mg L-1 BA. The differentiated shoots were further proliferated in the same medium, rooted in the presence of 0.5 mg L-1 NAA and hardened to grow in the field. Genetic variability in the form of a solid mutant of yellow colour has been obtained from this cultivar, when the freshly inoculated ray florets were treated with 1.0 Gy -radiation dose. |
| Page(s): | 121-124 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(1) [January 2007]
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