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Title: Standardization of <i style="">in vitro</i> protocol in <i style="">Chrysanthemum</i> cv. Madam E Roger for development of quality planting material and to induce genetic variability using <img src='/image/spc_char/gamma2.gif'>-radiation
Authors: Misra, Pratibha
Datta, S K
Keywords: <i style="">Chrysanthemum</i>
<i style="">in vitro</i> mutagenesis
quality planting material
shoot differentiation
<img src='/image/spc_char/gamma2.gif'>-radiation
ray florets
Issue Date: Jan-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00, 5/02; C12N15/01
Abstract: ‘Madam E Roger’, a greenish white large flowered chrysanthemum cultivar- was selected for <i style="">in vitro</i> propagation and mutagenesis to induce further genetic variability. A protocol has been standardized to develop large-scale quality planting material for commercial exploitation. Shoot bud differentiation could be achieved in ray florets in the presence of 0.5 mg L<sup>-1</sup> NAA+ 2.0 mg L<sup>-1</sup> BA. The differentiated shoots were further proliferated in the same medium, rooted in the presence of 0.5 mg L<sup>-1</sup> NAA and hardened to grow in the field. Genetic variability in the form of a solid mutant of yellow colour has been obtained from this cultivar, when the freshly inoculated ray florets were treated with 1.0 Gy <img src='/image/spc_char/gamma2.gif'>-radiation dose.
Description: 121-124
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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