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Title: Standardization of in vitro protocol in Chrysanthemum cv. Madam E Roger for development of quality planting material and to induce genetic variability using -radiation
Authors: Misra, Pratibha
Datta, S K
Keywords: Chrysanthemum
in vitro mutagenesis
quality planting material
shoot differentiation
ray florets
Issue Date: Jan-2007
Publisher: CSIR
Series/Report no.: Int. Cl.8 A01H4/00, 5/02; C12N15/01
Abstract: ‘Madam E Roger’, a greenish white large flowered chrysanthemum cultivar- was selected for in vitro propagation and mutagenesis to induce further genetic variability. A protocol has been standardized to develop large-scale quality planting material for commercial exploitation. Shoot bud differentiation could be achieved in ray florets in the presence of 0.5 mg L-1 NAA+ 2.0 mg L-1 BA. The differentiated shoots were further proliferated in the same medium, rooted in the presence of 0.5 mg L-1 NAA and hardened to grow in the field. Genetic variability in the form of a solid mutant of yellow colour has been obtained from this cultivar, when the freshly inoculated ray florets were treated with 1.0 Gy -radiation dose.
Description: 121-124
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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