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Title: Direct regeneration from apical bud explants of <i style="">Withania somnifera</i> Dunal
Authors: Sivanesan, I
Keywords: apical bud explants
direct regeneration
endangered plants
<i style="">Withania somnifera</i>
Issue Date: Jan-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> A01H4/00, 5/00
Abstract: Effecient multiplication of <i style="">Withania somnifera </i>was achieved through culture of shoots tips of mature plants on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Gamborg (B<sub>5</sub>) media. However, MS medium was found superior to SH and B<sub>5</sub>. MS medium supplemented with BAP+IAA (each at 2.0 mg/L) was optimal for induction of shoot buds; whereas, MS supplemented with 0.3 mg/L GA<sub>3</sub> was the most suitable for shoot elongation. Elongated shoots were rooted on half strength MS containing with 2.0 mg/L IBA. The rooted plantlets were successfully established in field.
Description: 125-127
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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