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IJBT Vol.06(1) [January 2007] >


Title: Direct regeneration from apical bud explants of Withania somnifera Dunal
Authors: Sivanesan, I
Keywords: apical bud explants
direct regeneration
endangered plants
micropropagation
Withania somnifera
Issue Date: Jan-2007
Publisher: CSIR
IPC CodeInt. Cl.8 A01H4/00, 5/00
Abstract: Effecient multiplication of Withania somnifera was achieved through culture of shoots tips of mature plants on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Gamborg (B5) media. However, MS medium was found superior to SH and B5. MS medium supplemented with BAP+IAA (each at 2.0 mg/L) was optimal for induction of shoot buds; whereas, MS supplemented with 0.3 mg/L GA3 was the most suitable for shoot elongation. Elongated shoots were rooted on half strength MS containing with 2.0 mg/L IBA. The rooted plantlets were successfully established in field.
Page(s): 125-127
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source: IJBT Vol.06(1) [January 2007]

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