Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5023
Title: Direct regeneration from apical bud explants of Withania somnifera Dunal
Authors: Sivanesan, I
Keywords: apical bud explants;direct regeneration;endangered plants;micropropagation;Withania somnifera
Issue Date: Jan-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00, 5/00
Abstract: Effecient multiplication of Withania somnifera was achieved through culture of shoots tips of mature plants on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Gamborg (B5) media. However, MS medium was found superior to SH and B5. MS medium supplemented with BAP+IAA (each at 2.0 mg/L) was optimal for induction of shoot buds; whereas, MS supplemented with 0.3 mg/L GA3 was the most suitable for shoot elongation. Elongated shoots were rooted on half strength MS containing with 2.0 mg/L IBA. The rooted plantlets were successfully established in field.
Page(s): 125-127
URI: http://hdl.handle.net/123456789/5023
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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