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Title: Initiation of embryogenic suspension culture and plant regeneration in onion (Allium cepa L.)
Authors: Tiwari, S
Tripathi, M K
Khare, U K
Rana, R
Keywords: Allium cepa;embryo culture;cell suspension culture;somatic embryo
Issue Date: Jan-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00, 5/10
Abstract: A rapidly growing and maintainable embryogenic suspension culture of Allium cepa L. was accomplished with two different approaches, either by culturing mature embryos directly or by transferring embryogenic callus cultures in liquid media. The cultures obtained were inundated with clumps of proliferating globular embryos with very little non-embryogenic tissues. The number and size of somatic embryo clumps was measured to quantify growth of embryogenic tissues under various conditions. The suspensions were subcultured every 15 d by replacing the old medium with an equal volume of fresh medium. Initiation and proliferation of such embryogenic suspension culture depended upon the genotypes, method of suspension culture initiation and various exogenous growth regulators supplemented to the culture medium at variable quantity. For the establishment of suspension cultures, the liquid MS media fortified with 4.0 mg L1 2,4-D in combination with 0.5 mg L-1 BAP was found to be the most effective. For subsequent subculturing, the reduced level of 2.0 mg L-1 2,4-D in combination with 0.5 mg L-1 BAP promoted faster development of embryos. Frequent and efficient plantlet regeneration occurred on MS medium with 0.5 mg.L-1 each of NAA, BAP and kinetin. Regenerated plants were found to be phenotypically normal and true to the type.
Page(s): 100-106
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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