Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5006
Title: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Expression of an antimicrobial peptide (MSI-99) confers enhanced resistance to <i style="">Aspergillus niger </i>in transgenic potato<i style=""></i> </smarttagtype></smarttagtype>
Authors: Ganapathi, T R
Ghosh, S B
Laxmi, N H S
Bapat, V A
Keywords: antimicrobial peptides
<i style="">Aspergillus niger</i>
MSI-99
transgenic potato
Issue Date: Jan-2007
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C12N15/09, 15/63
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> MSI-99, a synthetic substitution analogue of antimicrobial peptide magainin, was used to impart enhanced resistance against fungal pathogen, <i style="">Aspergillus niger</i> in transgenic potato cultivars, Kufri Jyothi and Kufri Bahar.<i style=""> </i>Internodal stem segments were transformed with <i style="">Agrobacterium tumefaciens</i> strain EHA105. Integration of the transgene, MSI-99 in the transgenic plants of both the cultivars was confirmed by PCR and PCR-Southern analysis, exhibiting 180 bp fragment and hybridization with labelled probe pSAN168. Bioassay studies using detached leaves of transgenics showed the enhanced resistance against <i style="">A. niger</i>. </smarttagtype></smarttagtype>
Description: 63-67
URI: http://hdl.handle.net/123456789/5006
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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