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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(1) [January 2007] >
| Title: | Expression of an antimicrobial peptide (MSI-99) confers enhanced resistance to Aspergillus niger in transgenic potato |
| Authors: | Ganapathi, T R
Ghosh, S B
Laxmi, N H S
Bapat, V A |
| Keywords: | antimicrobial peptides
Aspergillus niger
MSI-99
transgenic potato |
| Issue Date: | Jan-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N15/09, 15/63 |
| Abstract: | MSI-99, a synthetic substitution analogue of antimicrobial peptide magainin, was used to impart enhanced resistance against fungal pathogen, Aspergillus niger in transgenic potato cultivars, Kufri Jyothi and Kufri Bahar. Internodal stem segments were transformed with Agrobacterium tumefaciens strain EHA105. Integration of the transgene, MSI-99 in the transgenic plants of both the cultivars was confirmed by PCR and PCR-Southern analysis, exhibiting 180 bp fragment and hybridization with labelled probe pSAN168. Bioassay studies using detached leaves of transgenics showed the enhanced resistance against A. niger. |
| Page(s): | 63-67 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.06(1) [January 2007]
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