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Title:  Expression of an antimicrobial peptide (MSI-99) confers enhanced resistance to Aspergillus niger in transgenic potato
Authors: Ganapathi, T R
Ghosh, S B
Laxmi, N H S
Bapat, V A
Keywords: antimicrobial peptides;Aspergillus niger;MSI-99;transgenic potato
Issue Date: Jan-2007
Publisher: CSIR
IPC Code: Int. Cl.8 C12N15/09, 15/63
Abstract:  MSI-99, a synthetic substitution analogue of antimicrobial peptide magainin, was used to impart enhanced resistance against fungal pathogen, Aspergillus niger in transgenic potato cultivars, Kufri Jyothi and Kufri Bahar. Internodal stem segments were transformed with Agrobacterium tumefaciens strain EHA105. Integration of the transgene, MSI-99 in the transgenic plants of both the cultivars was confirmed by PCR and PCR-Southern analysis, exhibiting 180 bp fragment and hybridization with labelled probe pSAN168. Bioassay studies using detached leaves of transgenics showed the enhanced resistance against A. niger.
Page(s): 63-67
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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