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Title: Polyamines stimulate shoot bud proliferation and multiplication in Achras sapota grown in culture
Authors: Purohit, S D
Singhvi, A
Nagori, R
Vyas, S
Keywords: Achras sapota var. Cricket Ball;axillary bud break;cotyledonary node;in vitro propagation;polyamines
Issue Date: Jan-2007
Publisher: CSIR
IPC Code: Int. Cl.8 A01H5/12
Abstract: In vitro shoot bud proliferation and multiplication was stimulated in shoot clusters of Achras sapota when polyamines were incorporated in the Schenk and Hildebrandt’s (SH) medium in combination with 6-benzylaminopurine (BA). Of the three polyamines–putrescine (Put), spermidine (Spd) and spermine (Spm), Put proved to be highly stimulatory. With Put at 0.1 mM concentration in combination with 8.87 µM BA, a little less than three-fold rate of shoot multiplication could be achieved while BA alone could not evoke such response. The stimulatory effect of Put was followed by Spm. The involvement of polyamines in the above response was confirmed by suppression of process by use of their biosynthesis specific inhibitors– -difluoromethylornithine (DFMO) and -difluoromethylarginine (DFMA). DFMO at 1.0 mM concentration caused more effective suppression of shoot proliferation as compared to DFMA at the same concentration. The inhibitory effect could be reversed by incorporation of additional polyamines in the medium. Stimulated shoot bud proliferation (more than that obtained with Put) was also observed when GA3 was incorporated in place of polyamines in the medium. However, its consistent use was not found beneficial in the long run.
Page(s): 85-90
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.06(1) [January 2007]

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