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Title: Anti-Herpes Simplex Virus (HSV-1 and HSV-2) activity of biogenic gold and silver nanoparticles using seaweed Sargassum wightii
Authors: Dhanasezhian, A.
Srivani, S.
Govindaraju, K.
Parija, Preetam
Sasikala, S.
Kumar, M.R. Ramesh
Keywords: Sargasum wightii;Gold nanoparticles;Silver nanoparticles;HSV;Cytopathic effect;Antiviral
Issue Date: Aug-2019
Publisher: NISCAIR-CSIR, India
Abstract: Biogenic gold (Au) and silver (Ag) nanoparticles were synthesized using seaweed Sargassum wightii (Sw) and their antiviral activity against Herpes Simplex Virus (HSV-1 and HSV-2) was evaluated. Efficacy of Sw-Au and Sw-Ag nanoparticles against HSV was estimated by the reduction of cytopathic effect (CPE) caused by HSV, which was observed in a dose-dependent manner. Sw-Au nanoparticles reduced 70% CPE of HSV-1 and HSV-2 at 10 μL and 25 μL, respectively, whereas 2.5 μL Sw-Ag nanoparticles effectively reduced 70% CPE of HSV-1 and HSV-2. Cytotoxicity was estimated in Vero cells by MTT assay. Sw-Au nanoparticles were significantly non-toxic in all the concentrations tested, whereas Sw-Ag nanoparticles were found to be toxic in higher concentrations. Sw-Au nanoparticles showed cell viability of 93.12-85.18 % in the range of 2.5-25 µL, and Sw-Ag nanoparticles showed cell viability of 97.21-21.91% in the range of 1-10 μL. Sw-Au and Sw-Ag nanoparticles effectively reduce the CPE caused by both HSV-1 and HSV-2 in Vero cells and can be used to treat HSV infections.
Page(s): 1252-1257
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.48(08) [August 2019]

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