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Title: Antifouling activity of alkaline protease from halotolerant Bacillus sp. isolated from marine source
Authors: Kavitha, G.
Inbakandan, D.
Nalini, S.
Riyaz, S. U. Mohammed
Keywords: Halobacteria;Antifouling;Protease;Algicide
Issue Date: Aug-2019
Publisher: NISCAIR-CSIR, India
Abstract: In this study, an attempt has been made to explore the antifouling activity of the marine halotolerant bacterium, namely, Bacillus sp. About 10 different bacteria were isolated using the Zobell marine agar medium from the marine sediment samples that were collected from the Marina beach, Bay of Bengal, India. The growth pattern tests of the 10 bacteria were documented. Among the 10 different isolates, two exhibited the protease activity in skim milk agar. The antifouling activity was further tested against five different fouling bacteria. The antifouling activity of alkaline protease isolated from Bacillus sp. inferred that it exhibited pronounced inhibitory zones against fouling bacterial strains with least minimal inhibitory concentration range between 25 and 100 units/mg protein. Anticrustacean assay was performed using Artemia salina of alkaline protease, which showed good activity. The results suggest that Bacillus sp. produced an extracellular alkaline protease, which was responsible for the antifouling and algicidal activity of marine fouling organisms. This study tends to indicate that Bacillus sp. could be further explored for the development of new antifouling agents.
Page(s): 1274-1279
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.48(08) [August 2019]

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