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Title: Biogenic synthesis of gold nanoparticles using Sargassum tenerrimum and its evaluation of antibacterial activity against Escherichia coli and Salmonella typhi
Authors: Dhas, T. Stalin
Kumar, V. Ganesh
AbdulAzeez, Sayed
Borgio, J. Francis
Karthick, V.
Keywords: Sargassum tenerrimum;Escherichia coli;Salmonella typhi;Gold nanoparticles;Biosynthesis;Electron microscopy;Antibacterial activity
Issue Date: Aug-2019
Publisher: NISCAIR-CSIR, India
Abstract: An important aspect of nanotechnology is the development of a reliable and ecofriendly method for the synthesis of nanomaterials. In the present study, Sargassum tenerrimum extract was used to synthesize gold nanoparticles (AuNPs) by the reduction of AuCl4 ions to Au0. The formation and morphology of the synthesized AuNPs were investigated using \UV–visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, and field emission scanning electron microscopy with energy dispersive X-ray analysis. The nanoparticles synthesized at 60 °C were spherical in shape while few were also hexagonal in shape with the size ranging between 10 and 40 nm. The antibacterial activity of AuNPs was tested against Escherichia coli (ATCC 25922) and Salmonella typhi (ATCC 6539). On examination under an epifluorescence microscope, it was found to cause a significant amount of deterioration to the bacterial cells.
Page(s): 1291-1297
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.48(08) [August 2019]

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