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Title: Bioprospecting of actinobacteria from the Andaman marine ecosystem: Isolation, antagonistic potential, and taxonomy of potential strain
Authors: Manisha, M.
Mithali, J.
Vijayalakshmi, G.
Gopikrishnan, V.
Masilamaniselvam, M.
Radhakrishnan, M.
Keywords: Streptomyces sp.;Antimicrobial activity;Mangrove sediments;Andaman Islands;MIC
Issue Date: Aug-2019
Publisher: NISCAIR-CSIR, India
Abstract: The present study reports on the bioprospecting of actinobacteria from the Andaman marine ecosystem with special reference to antimicrobial compounds. Twenty-eight morphologically different actinobacterial cultures were isolated from rhizosphere sediments collected from five mangrove plants in Dhaninallah, Middle Andaman. Twenty out of 28 actinobacterial strains showed antagonistic activity in which 13 strains showed broad-spectrum activity against Gram positive and Gram negative bacterial pathogens. Strain AMA37, which showed the maximum zone of inhibition, was selected as the potential strain for further studies. Bioactive metabolites were produced by solid-state fermentation and the methanol extracts of AMA 37 showed broad-spectrum activity against clinical, fish, poultry, and fungal pathogens. The MIC (minimal inhibitory concentration) of crude extract ranged between 62.5 and 250 µg/ml. On the basis of phenotypic and molecular characteristics, the strain AMA37 was identified as a novel species of the genus Streptomyces as it showed 97% similarity with the closely related strain Streptomyces djakartensis. The 16s rRNA sequence was deposited in GenBank with accession number KU999997. The present study revealed that Streptomyces sp. AMA37 is a promising source for isolation of bioactive compounds and should pave the way for clinical, poultry, and aquaculture applications.
Page(s): 1312-1318
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.48(08) [August 2019]

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