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|Title:||Regulation of Process Parameters for Improved Synthesis of Thrombolytic Enzyme from Bacillus cereus S46|
|Authors:||D’Souza, D H|
|Keywords:||Bacillus cereus;Thrombolytic enzyme;Submerged fermentation;Process optimization|
|Abstract:||The study aimed to isolate soil bacteria and screen them for thrombolytic activity. Effect of process parameters on enzyme production were analyzed using one factor at a time approach. Isolate S46 demonstrated highest blood clot dissolution (15.64%) and was chosen for further study. Based on the partial 16S rDNA sequence, isolate S46 was identified as Bacillus cereus. Addition of sucrose and casein (1% w/v) to the broth resulted in highest enzyme yield (130 U/ml). Enzyme production improved in presence of MgSO4 (0.08%, w/v), FeSO4 (0.004% w/v), respectively. Enzyme production was best (186.66 and 210.86 U/ml) at pH 8.0 and 30 °C. Maximum enzyme production (220.86 U/ml) was reported at 72 h with inoculum size of 1.5% v/v. The above conditions advocate that media optimization enhanced the thrombolytic enzyme yield by Bacillus cereus S46.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.78(07) [July 2019]|
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