Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/48785
Title: Regulation of Process Parameters for Improved Synthesis of Thrombolytic Enzyme from Bacillus cereus S46
Authors: D’Souza, D H
Bhattacharya, S
Keywords: Bacillus cereus;Thrombolytic enzyme;Submerged fermentation;Process optimization
Issue Date: Jul-2019
Publisher: NISCAIR-CSIR, India
Abstract: The study aimed to isolate soil bacteria and screen them for thrombolytic activity. Effect of process parameters on enzyme production were analyzed using one factor at a time approach. Isolate S46 demonstrated highest blood clot dissolution (15.64%) and was chosen for further study. Based on the partial 16S rDNA sequence, isolate S46 was identified as Bacillus cereus. Addition of sucrose and casein (1% w/v) to the broth resulted in highest enzyme yield (130 U/ml). Enzyme production improved in presence of MgSO4 (0.08%, w/v), FeSO4 (0.004% w/v), respectively. Enzyme production was best (186.66 and 210.86 U/ml) at pH 8.0 and 30 °C. Maximum enzyme production (220.86 U/ml) was reported at 72 h with inoculum size of 1.5% v/v. The above conditions advocate that media optimization enhanced the thrombolytic enzyme yield by Bacillus cereus S46.
Page(s): 442-447
URI: http://nopr.niscair.res.in/handle/123456789/48785
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.78(07) [July 2019]

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