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Title: An efficient <i>in vitro</i> protocol for clonal multiplication of Ginger – var. Varada
Authors: Kavyashree, R
Keywords: Vegetative bud
<i>in vitro</i> multiplication
survival frequency
<i>Zingiber officinale</i>
Issue Date: Jul-2009
Publisher: CSIR
Abstract: An efficient, <i style="">in vitro </i>multiplication protocol was developed for <i style="">Zingiber officinale </i>Rosc., var. Varada through direct regeneration of vegetative buds. Multiple shoots were induced from vegetative buds on LSBM fortified with BAP (17.76 μ<i style="">M</i>) with 96% initiation response. The repeated subculture resulted in rapid shoot multiplication at the average rate of 4-fold per culture. The well-developed multiple shoots formed roots on the same medium after 2-3 passages of subculture, thus eliminating the step of <i style="">in vitro </i>rooting. The statistical data pertaining to multiple shoot and root formation revealed highest mean number of 19.1 and 12.3, respectively. The regenerated plantlets were successfully established in the field with 86% survival frequency after few days of indoor acclimatization.
Description: 328-331
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(3) [July 2009]

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