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Title: Growth and shoot proliferation in <i style="">Chlorophytum borivilianum </i>Sant. et Fernand. <i style="">in vitro</i> under different carbon dioxide environment
Authors: Joshi, N
Dave, A
Vyas, S
Purohit, S D
Keywords: Carbon dioxide enrichment
<i style="">Chlorophytum borivilianum</i>
<i style="">in vitro</i> growth
shoot culture
Issue Date: Jul-2009
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> <i style="">In vitro</i> growth and shoot proliferation in<i style=""> Chlorophytum borivilianum </i>Sant. et Fernand. were studied in a controlled CO<sub>2</sub> environment. The cultures were grown on BA supplemented MS medium with or without 3% sucrose. A range of CO<sub>2</sub> concentrations (0.0, 0.6, 10.0 and 40.0 g m<sup>-3</sup>) was controlled in small chambers by using solutions of NaHCO<sub>3</sub>, Na<sub>2</sub>CO<sub>3</sub>, KHCO<sub>3</sub> and K<sub>2</sub>CO<sub>3</sub>. In order to maintain a CO<sub>2</sub>-free environment, a saturated solution of KOH was kept in the chambers. It was observed that the growing shoot cultures required either sucrose in the medium as a carbon source or an environment with controlled CO<sub>2</sub> concentration. Complete absence of a carbon source caused severe yellowing of shoots and death within 15 d. The growth of shoot cultures at 40.0 g m<sup>-3</sup> CO<sub>2 </sub>was comparable to the growth obtained with 3.0% sucrose in the medium. With both CO<sub>2</sub> and sucrose being available, the best response was obtained at 40.0 g m<sup>-3</sup> CO<sub>2</sub> in the chamber. At this concentration the increase in number of shoots was nearly double the standard rate obtained when exposed to the natural CO<sub>2</sub> level and sucrose supplemented medium. Total fresh and dry wt and shoot number were the maximum under this condition. </smarttagtype>
Description: 323-327
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(3) [July 2009]

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