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Title: Growth and shoot proliferation in Chlorophytum borivilianum Sant. et Fernand. in vitro under different carbon dioxide environment
Authors: Joshi, N
Dave, A
Vyas, S
Purohit, S D
Keywords: Carbon dioxide enrichment
Chlorophytum borivilianum
in vitro growth
micropropagation
photoautotrophy
shoot culture
Issue Date: Jul-2009
Publisher: CSIR
Abstract:  In vitro growth and shoot proliferation in Chlorophytum borivilianum Sant. et Fernand. were studied in a controlled CO2 environment. The cultures were grown on BA supplemented MS medium with or without 3% sucrose. A range of CO2 concentrations (0.0, 0.6, 10.0 and 40.0 g m-3) was controlled in small chambers by using solutions of NaHCO3, Na2CO3, KHCO3 and K2CO3. In order to maintain a CO2-free environment, a saturated solution of KOH was kept in the chambers. It was observed that the growing shoot cultures required either sucrose in the medium as a carbon source or an environment with controlled CO2 concentration. Complete absence of a carbon source caused severe yellowing of shoots and death within 15 d. The growth of shoot cultures at 40.0 g m-3 CO2 was comparable to the growth obtained with 3.0% sucrose in the medium. With both CO2 and sucrose being available, the best response was obtained at 40.0 g m-3 CO2 in the chamber. At this concentration the increase in number of shoots was nearly double the standard rate obtained when exposed to the natural CO2 level and sucrose supplemented medium. Total fresh and dry wt and shoot number were the maximum under this condition.
Page(s): 323-327
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(3) [July 2009]

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