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Title: Influence of biotic and abiotic elicitors on accumulation of hyoscyamine and scopolamine in root cultures of<i> Datura metel</i> L.<b></b>
Authors: Ajungla, L
Patil, P P
Barmukh, R B
Nikam, T D
Keywords: <i>Datura metel</i>
tropane alkaloids
Issue Date: Jul-2009
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region" downloadurl=""><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl=""><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> Leaf-derived root cultures of <i>Datura metel</i> L., established on B5 medium containing 1.2 µ<i>M</i> IAA, were employed to study the influence of biotic (<i>Aspergillus niger, Alternaria </i>sp., <i>Fusarium monoliforme</i> and yeast extract) and abiotic (salicylic acid, AlCl<sub>3</sub>, CaCl<sub>2</sub>, NaCl and Na<sub>2</sub>SO<sub>4</sub>) elicitors on the growth and production of hyoscyamine and scopolamine, the medicinally important tropane alkaloids. The hyoscyamine and scopolamine contents in control root cultures were 1.39 mg/g dw and 0.069 mg/g dw, respectively. The highest hyoscyamine (4.35 mg/g dw) and scopolamine (0.28 mg/g dw) accumulation was obtained in cultures treated with 500 μ<i>M</i> salicylic acid, followed by treatment with 0.75 g L<sup>-1</sup> yeast extract (3.17 mg/g dw hyoscyamine & 0.16 mg/g dw scopolamine) and 250 μ<i>M </i>AlCl<sub>3</sub> (2.49 mg/g dw hyoscyamine and 0.11 mg/g dw scopolamine). </smarttagtype></smarttagtype></smarttagtype>
Description: 317-322
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(3) [July 2009]

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