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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(3) [July 2009] >
| Title: | In vitro propagation of Citrus indica Tanaka—An endangered progenitor species |
| Authors: | Laskar, M A
Hynniewta, M
Rao, C S |
| Keywords: | Citrus indica endangered
ex situ adaptibility
leaf-derived callus
progenitor
shoot organogenesis |
| Issue Date: | Jul-2009 |
| Publisher: | CSIR |
| Abstract: | A method for in vitro propagation of Citrus indica Tanaka by shoot organogenesis from leaf-derived callus was developed. Regenerative calli were induced on MS medium supplemented with 0.01 mg L‑1 TDZ and 0.1 mg L‑1 NAA. Shoots were regenerated on WPM medium supplemented with 0.5 mg L‑1 BAP, 0.25 mg L‑1 TDZ and 0.25 mg L‑1 NAA. Regenerated shoots were rooted on MS medium supplemented with 1.0 mg L‑1 NAA. Sixty per cent of the rooted plantlets were acclimatized successfully under ex situ conditions. |
| Page(s): | 311-316 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(3) [July 2009]
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