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Title: <i>In vitro</i> propagation of <i>Citrus indica </i>Tanaka—An endangered progenitor species
Authors: Laskar, M A
Hynniewta, M
Rao, C S
Keywords: <i>Citrus indica</i> endangered
<i style="">ex situ</i> adaptibility
leaf-derived callus
shoot organogenesis
Issue Date: Jul-2009
Publisher: CSIR
Abstract: A method for <i>in vitro</i> propagation of <i style="">Citrus indica</i> Tanaka by shoot organogenesis from leaf-derived callus was developed. Regenerative calli were induced on MS medium supplemented with 0.01 mg L<sup>‑1</sup> TDZ and 0.1 mg L<sup>‑1</sup> NAA. Shoots were regenerated on WPM medium supplemented with 0.5 mg L<sup>‑1</sup> BAP, 0.25 mg L<sup>‑1</sup> TDZ and 0.25 mg L<sup>‑1</sup> NAA. Regenerated shoots were rooted on MS medium supplemented with 1.0 mg L<sup>‑1</sup> NAA. Sixty per cent of the rooted plantlets were acclimatized successfully under <i>ex situ</i> conditions.
Description: 311-316
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(3) [July 2009]

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