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IJBT Vol.08(3) [July 2009] >


Title: In vitro propagation of Citrus indica Tanaka—An endangered progenitor species
Authors: Laskar, M A
Hynniewta, M
Rao, C S
Keywords: Citrus indica endangered
ex situ adaptibility
leaf-derived callus
progenitor
shoot organogenesis
Issue Date: Jul-2009
Publisher: CSIR
Abstract: A method for in vitro propagation of Citrus indica Tanaka by shoot organogenesis from leaf-derived callus was developed. Regenerative calli were induced on MS medium supplemented with 0.01 mg L‑1 TDZ and 0.1 mg L‑1 NAA. Shoots were regenerated on WPM medium supplemented with 0.5 mg L‑1 BAP, 0.25 mg L‑1 TDZ and 0.25 mg L‑1 NAA. Regenerated shoots were rooted on MS medium supplemented with 1.0 mg L‑1 NAA. Sixty per cent of the rooted plantlets were acclimatized successfully under ex situ conditions.
Page(s): 311-316
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(3) [July 2009]

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