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Title: Biopolymer based intervention for <i>Salmonella </i>in water
Authors: Ghosh, Moushumi
Pathak, Santosh
Keywords: Biopolymer
<i style="">Klebsiella terrigena</i>
<i style="">Salmonella</i> <i style="">typhimurium</i>
Issue Date: Jul-2009
Publisher: CSIR
Abstract: This study evaluated the potential of a bacterial polymer for binding and removal of <i style="">Salmonella</i> spp. currently deemed as a biowarfare agent in water. The biopolymer previously characterized as a polysaccharide with flocculating activity is produced extracellularly by the bacterium,<i style=""> Klebsiella terrigena</i>. The biopolymer could effectively remove 3 log cfu/mL of <i style="">Salmonella typhimurium</i> ATCC 23564, within a period of 30 min from amongst other indicator (<i style="">Escherichia coli</i> DH5α and <i style="">Enterococcus faecalis</i> ATCC 35550) and enteropathogens of moderate concern (<i style="">Staphylococcus aureus</i> ATCC 9144) spiked in water samples at ambient temperature. The optimum dose of biopolymeric flocculant for removal of <i style="">Salmonella </i>was 2 mg/L. FISH and CLSM demonstrated that the removed <i style="">S. typhimurium</i> was selectively bound to the biopolymer matrix. The results indicate a significant possibility of using this biopolymer for rapid detection and removal of key biowarfare agents (for instance <i style="">Salmonella</i>) transmitted through water.
Description: 298-303
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(3) [July 2009]

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