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IJBT Vol.08(3) [July 2009] >


Title: Biopolymer based intervention for Salmonella in water
Authors: Ghosh, Moushumi
Pathak, Santosh
Keywords: Biopolymer
biowarfare
flocculation
polysaccharide
Klebsiella terrigena
Salmonella typhimurium
Issue Date: Jul-2009
Publisher: CSIR
Abstract: This study evaluated the potential of a bacterial polymer for binding and removal of Salmonella spp. currently deemed as a biowarfare agent in water. The biopolymer previously characterized as a polysaccharide with flocculating activity is produced extracellularly by the bacterium, Klebsiella terrigena. The biopolymer could effectively remove 3 log cfu/mL of Salmonella typhimurium ATCC 23564, within a period of 30 min from amongst other indicator (Escherichia coli DH5α and Enterococcus faecalis ATCC 35550) and enteropathogens of moderate concern (Staphylococcus aureus ATCC 9144) spiked in water samples at ambient temperature. The optimum dose of biopolymeric flocculant for removal of Salmonella was 2 mg/L. FISH and CLSM demonstrated that the removed S. typhimurium was selectively bound to the biopolymer matrix. The results indicate a significant possibility of using this biopolymer for rapid detection and removal of key biowarfare agents (for instance Salmonella) transmitted through water.
Page(s): 298-303
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(3) [July 2009]

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