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IJBT Vol.08(3) [July 2009] >

Title: Optimization of protease production from newly isolated strain of Bacillus sp. PCSIR EA-3
Authors: Qadar, Shah Ali Ul
Shireen, Erum
Iqbal, Samina
Anwar, Abida
Keywords: Bacillus sp.
calcium ion
culture optimization
Issue Date: Jul-2009
Publisher: CSIR
Abstract: A newly isolated strain of Bacillus sp. PCSIR EA-3 showed maximum protease production in 48 h. Cell free filtrate (CFF) from the strain was investigated for its proteolytic activity. The isolated strain exhibited highest proteolytic activity when grown on skim milk agar; average area of zone of inhibition was 480 mm2. Enzyme showed maximum activity at 35°C and pH 7.0 with glucose as an important medium component. It was strongly activated by metal ions, such as, Ca+2 ion. Protease from PCSIR EA-3 can easily be used in food industry, especially in cheese manufacturing due to its thermostability at neutral pH.
Page(s): 286-290
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(3) [July 2009]

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