NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(3) [July 2009] >


Title: Optimization of protease production from newly isolated strain of Bacillus sp. PCSIR EA-3
Authors: Qadar, Shah Ali Ul
Shireen, Erum
Iqbal, Samina
Anwar, Abida
Keywords: Bacillus sp.
calcium ion
culture optimization
protease
Issue Date: Jul-2009
Publisher: CSIR
Abstract: A newly isolated strain of Bacillus sp. PCSIR EA-3 showed maximum protease production in 48 h. Cell free filtrate (CFF) from the strain was investigated for its proteolytic activity. The isolated strain exhibited highest proteolytic activity when grown on skim milk agar; average area of zone of inhibition was 480 mm2. Enzyme showed maximum activity at 35°C and pH 7.0 with glucose as an important medium component. It was strongly activated by metal ions, such as, Ca+2 ion. Protease from PCSIR EA-3 can easily be used in food industry, especially in cheese manufacturing due to its thermostability at neutral pH.
Page(s): 286-290
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(3) [July 2009]

Files in This Item:

File Description SizeFormat
IJBT 8(3) 286-290.pdf176.36 kBAdobe PDFView/Open
 Current Page Visits: 927 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 586431 since 06-Feb-2009  Last updated on 18-Sep-2014Webmaster: nopr@niscair.res.in