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Title: Spectro-analytical and in vitro biological studies of novel nalidixic acid hydrazone and its transition metal complexes
Authors: Narsimha, Nagula
Jaheer, Mohmed
Reddy, Palreddy Ranjith
Sudeepa, Kunche
Devi, Ch. Sarala
Keywords: Nalidixic acid;Antimicrobial activity;DNA interactions;MTT assay;Molecular docking
Issue Date: Apr-2019
Publisher: NISCAIR-CSIR, India
Abstract: The novel and potential chelating agent derived from Nalidixic acid and its binary Cu(II), Ni(II) and Co(II) metal complexes have been synthesized and characterized by elemental analyses, 1H-NMR, Mass, UV-vis, FT-IR, TGA, SEM-EDX, ESR and magnetic susceptibility measurements. All the systems of the present study have been screened for antimicrobial activity against bacterial species Bacillus (Gram +) and Escherichia coli (Gram -) and fungal species Sclerotium rolfsii and Macrophomina phaseolina exhibited an enhanced activity of metal complexes over unbound free chelating agent. The interaction studies of metal complexes with Calf-thymus DNA (CT-DNA) investigated by UV-visible and fluorescence titrations revealed the intercalation mode of binding. Further MTT assay performed on HeLa (human cervical cancer) and A549 (human lung tumor) cell lines revealed the IC50 values for the studied title compounds. In addition computer-aided molecular docking technique carried out with all title compounds employing molecular target DNA (PDB ID: 1N37), furnished docking scores and mode of binding for geometry optimized molecular structures of title compounds.
Page(s): 436-445
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.58A(04) [April 2019]

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