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Title: Interaction of Ni(II)-ethylenediamine/histamine with histidylglycine and investigation of their DNA cleavage abilities
Authors: Reddy, Pulimamidi R
Raju, Nomula
Rao, Kandibanda S
Shilpa, Addla
Keywords: Bioinorganic chemistry
Solution chemistry
Stability constants
DNA binding
DNA cleavage
Nickel (II)
Issue Date: Jun-2009
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>8</sup> C07F15/04
Abstract: The interaction of Ni(II)-ethylenediamine (en)/histamine(hist) with histidylglycine(his-gly) and their DNA cleavage abilities have been investigated. The stability constants for the Ni(II)-en-his-gly (<b>1</b>) and Ni(II)-hist-his-gly (<b>2</b>) complexes have been determined at 298.0 K and 0.10 <i style="">M </i>(KNO<sub>3</sub>) ionic strength. Both the 1:1:1 ternary complexes have been corroborated by species distribution curves and ESI-MS analysis. The complex geometries have been analyzed by UV-vis experiments and molecular mechanics simulations. The DNA binding abilities of these complexes have been established by UV-vis-absorption, thermal-denaturation and fluorescence spectroscopy. The intrinsic binding constants for the bound complexes, Ni(II)-en-his-gly:DNA and Ni(II)-hist-his-gly:DNA, have been determined to be 280 and 420 <i style="">M</i><sup>-1</sup> respectively. Gel-electrophoresis experiments reveal that <b>1</b> and <b>2</b> cleave supercoiled DNA (type-I) to the nicked-circular (type-II) form hydrolytically at physiological <i style="">p</i>H. A tentative mechanism is proposed for the cleavage.
Description: 761-768
ISSN: O975-0975 (Online); 0376-4710 (Print)
Appears in Collections:IJC-A Vol.48A(06) [June 2009]

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