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Title: Interaction of Ni(II)-ethylenediamine/histamine with histidylglycine and investigation of their DNA cleavage abilities
Authors: Reddy, Pulimamidi R
Raju, Nomula
Rao, Kandibanda S
Shilpa, Addla
Keywords: Bioinorganic chemistry;Solution chemistry;Stability constants;DNA binding;DNA cleavage;Nickel (II)
Issue Date: Jun-2009
Publisher: CSIR
IPC Code: Int. Cl.8 C07F15/04
Abstract: The interaction of Ni(II)-ethylenediamine (en)/histamine(hist) with histidylglycine(his-gly) and their DNA cleavage abilities have been investigated. The stability constants for the Ni(II)-en-his-gly (1) and Ni(II)-hist-his-gly (2) complexes have been determined at 298.0 K and 0.10 M (KNO3) ionic strength. Both the 1:1:1 ternary complexes have been corroborated by species distribution curves and ESI-MS analysis. The complex geometries have been analyzed by UV-vis experiments and molecular mechanics simulations. The DNA binding abilities of these complexes have been established by UV-vis-absorption, thermal-denaturation and fluorescence spectroscopy. The intrinsic binding constants for the bound complexes, Ni(II)-en-his-gly:DNA and Ni(II)-hist-his-gly:DNA, have been determined to be 280 and 420 M-1 respectively. Gel-electrophoresis experiments reveal that 1 and 2 cleave supercoiled DNA (type-I) to the nicked-circular (type-II) form hydrolytically at physiological pH. A tentative mechanism is proposed for the cleavage.
Page(s): 761-768
ISSN: O975-0975 (Online); 0376-4710 (Print)
Appears in Collections:IJC-A Vol.48A(06) [June 2009]

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