|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.48A [2009] >
IJC-A Vol.48A(06) [June 2009] >
| Title: | Interaction of Ni(II)-ethylenediamine/histamine with histidylglycine and investigation of their DNA cleavage abilities |
| Authors: | Reddy, Pulimamidi R
Raju, Nomula
Rao, Kandibanda S
Shilpa, Addla |
| Keywords: | Bioinorganic chemistry
Solution chemistry
Stability constants
DNA binding
DNA cleavage
Nickel (II) |
| Issue Date: | Jun-2009 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C07F15/04 |
| Abstract: | The interaction of Ni(II)-ethylenediamine (en)/histamine(hist) with histidylglycine(his-gly) and their DNA cleavage abilities have been investigated. The stability constants for the Ni(II)-en-his-gly (1) and Ni(II)-hist-his-gly (2) complexes have been determined at 298.0 K and 0.10 M (KNO3) ionic strength. Both the 1:1:1 ternary complexes have been corroborated by species distribution curves and ESI-MS analysis. The complex geometries have been analyzed by UV-vis experiments and molecular mechanics simulations. The DNA binding abilities of these complexes have been established by UV-vis-absorption, thermal-denaturation and fluorescence spectroscopy. The intrinsic binding constants for the bound complexes, Ni(II)-en-his-gly:DNA and Ni(II)-hist-his-gly:DNA, have been determined to be 280 and 420 M-1 respectively. Gel-electrophoresis experiments reveal that 1 and 2 cleave supercoiled DNA (type-I) to the nicked-circular (type-II) form hydrolytically at physiological pH. A tentative mechanism is proposed for the cleavage. |
| Page(s): | 761-768 |
| ISSN: | O975-0975 (Online); 0376-4710 (Print) |
| Source: | IJC-A Vol.48A(06) [June 2009]
|
|
