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Title: Improved and convenient method of RNA isolation from polyphenols and polysaccharide rich plant tissues
Authors: Kansal, Rekha
Kuhar, Kalika
Verma, Isha
Gupta, Ram Niwas
Gupta, Vijay Kumar
Koundal, Kirpa Ram
Keywords: cDNA synthesis;Leguminous seeds;RNA isolation
Issue Date: Dec-2008
Publisher: CSIR
Abstract: It has been difficult to extract a good quality total RNA from the plant parts (such as seeds) which contain high levels of phenolic compounds, carbohydrates and other compounds that bind and/or co-precipitate with RNA. A simple, rapid and efficient method for isolating total RNA from polyphenols and polysaccharide rich plant tissues has been developed. Seeds of leguminosae family were chosen for the study. The good quality and high yield of total RNA was achieved with A260/A280 ratio of 1.9. Seeds of three different crops (Cajanus cajan, Dolichos biflorus and Vigna mungo) at different developmental stages were evaluated for total RNA extraction using standardized protocol. Seeds at 21 days after flowering (DAF) gave the best results among others (7 DAF and dry seeds). Quality of isolated RNA from all the three crops was further checked by cDNA synthesis. The extracted RNA was found suitable for further molecular applications such as reverse transcription and cDNA library construction.
Page(s): 842-845
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.46(12) [December 2008]

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