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Title: Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles
Authors: Hema, M
Reddy, Ch V Subba
Savithri, H S
Sreenivasulu, P
Keywords: Potyvirus-like particles;Recombinant coat protein;Sugarcane streak mosaic virus
Issue Date: Nov-2008
Publisher: CSIR
Abstract: Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
Page(s): 793-796
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.46(11) [November 2008]

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