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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.46 [2008] >
IJEB Vol.46(11) [November 2008] >
| Title: | Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles |
| Authors: | Hema, M
Reddy, Ch V Subba
Savithri, H S
Sreenivasulu, P |
| Keywords: | Potyvirus-like particles
Recombinant coat protein
Sugarcane streak mosaic virus |
| Issue Date: | Nov-2008 |
| Publisher: | CSIR |
| Abstract: | Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization. |
| Page(s): | 793-796 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.46(11) [November 2008]
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