Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/4634
Title: Cell culture adapted sheeppox virus as a challenge virus for potency testing of sheeppox vaccine
Authors: Hosamani, M
Bhanuprakash, V
Kallesh, D J
Balamurugan, V
Pande, A
Singh, R K
Keywords: Cell culture
Potency testing
Sheeppox vaccine
Sheeppox virus
Virulent virus challenge
Issue Date: Oct-2008
Publisher: CSIR
Abstract:  Sheeppox virus from an outbreak of sheeppox that occurred in Srinagar (Jammu and Kashmir, India) in 2000 was isolated by inoculation of susceptible sheep and further re-isolated in cell culture. The field virus, adapted to grow in lamb testes culture, was evaluated for its potential use as challenge virus in potency testing of sheeppox vaccine currently in use. The virus (passage 6) produced severe disease in susceptible sheep when inoculated subcutaneously with a dose of 106.2 TCID50. The virus identity was confirmed by PCR, sequencing of P32 gene and species-specific signature residues identified in deduced aa sequence of the gene. The virus was successfully evaluated for its virulence using two batches of sheep pox vaccines. Use of this field virus enables consistent potency experiments of sheeppox vaccines avoiding use of animals for its propagation and titration.
Description: 685-689
URI: http://hdl.handle.net/123456789/4634
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.46(10) [October 2008]

Files in This Item:
File Description SizeFormat 
IJEB 46(10) 685-689.pdf209.79 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.