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|Title:||Protease, lipase and amylase extraction and optimization from activated sludge of pulp and paper industry|
|Authors:||Karn, Santosh Kumar|
|Keywords:||Cation exchange resin;Hydrolytic enzymes;Industrial sludge;Pollution;Triton X-100|
|Abstract:||Sludge is a hazardous waste and their disposal is an issue of concern that needs a suitable solution. Enzyme extraction from industrial sludge, particularly paper and pulp industry, is important in terms of waste management as well as the recovery of valuable product for the multifarious application. Present research work is focused on the extraction of some hydrolytic enzymes (protease, lipase, and amylase) from the sludge of paper industry using ultrasonication, an addition of cation exchange resin (CER) and Triton X-100. We have found that maximum protease was recovered from sludge using 2% Triton X-100 and maximum lipase at 0.1% Triton X-100. Both enzymes have shown contrasting behaviour. Lipase recovery was decreased with increased Triton X-100 concentration in contrast to protease recovery which showed a significant increase. However, maximum amylase was recovered using CER at 2000 U/g of VSS (volatile suspended solid) with Triton X-100 and maximum recovery was observed at 1.0%. Results suggest that this method to be most appropriate for extraction of hydrolytic enzymes from the activated sludge and suitable to be scaled up for effective recovery of hydrolytic enzymes from the industrial sludge.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.57(03) [March 2019]|
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