Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/4589
Title: Buffering capacity and membrane H<sup>+</sup> conductance of protease producing facultative alkaliphilic bacterium <i>Bacillus </i><i style="">flexus</i> from mangrove soil
Authors: Kannan, P
Ignacimuthu, S
Paulraj, M Gabriel
Keywords: <i style="">Bacillus flexus</i>
Buffering capacity
Facultative alkaliphile
Membrane H<sup>+</sup> conductance
Protease
Issue Date: Jun-2009
Publisher: CSIR
Abstract: A facultative alkaliphilic protease-producing gram-positive rod-shaped bacteria (EMGA 5) was isolated from mangrove soil and confirmed as <i style="">Bacillus flexus</i> by the 16S rDNA sequence. Buffering capacity and membrane H<sup>+</sup> conductance of this alkaliphilic isolate were investigated for the cells grown at <i style="">p</i>H 7.2 and 10.5 using acid pulse technique. Suspensions of <i>B. flexus </i>cells grown in poly peptone yeast glucose medium at <i style="">p</i>H 10.5 exhibited higher cytoplasmic membrane buffering capacity values (70 µmol H<sup>+</sup>/<i>p</i>H unit/mg protein at <i style="">p</i>H 9.9) than the cells grown at <i style="">p</i>H 7.2 (41 µmol H<sup>+</sup>/<i style="">p</i>H unit/mg protein at <i style="">p</i>H 9.9). <i>B. flexus </i>grown aerobically at <i style="">p</i>H 7.2 showed higher H<sup>+</sup> conductance values than the cells grown at <i style="">p</i>H 10.5 (0.032 µmol H<sup>+</sup>/s/<i style="">p</i>H unit/mg protein at <i style="">p</i>H 9.9 and 0.028 µmol H<sup>+</sup>/s/<i style="">p</i>H unit/mg protein at <i style="">p</i>H 9.8, respectively). The present study revealed that the buffering capacity and membrane H<sup>+</sup> conductance of the <i>B. flexus </i>isolates were influenced by <i style="">p</i>H of the medium.
Description: 261-265
URI: http://hdl.handle.net/123456789/4589
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.46(3) [June 2009]

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