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Title: Antioxidant effect of ethanolic extract of Piper betle Linn. (Paan) on erythrocytes from patients with HbE-beta thalassemia
Authors: Srimani, Phalguni
Mandal, Goutam
Ganguly, Sudipto
Saha, Piu
Sen, Rupashree
De, Rajib
Chatterjee, Arnab
Bhattacharyya, Maitrayee
Chaudhuri, Utpal
Bandyopadhyay, Susanta K
Chatterjee, Mitali
Keywords: HbE beta thalassemia
Reactive oxygen species (ROS)
Piper betle Linn
Anti-oxidant
Erythrocytes
Issue Date: Jun-2009
Publisher: CSIR
Abstract: HbE-beta thalassemia is caused by an interaction between HbE and defective b globin gene of thalassemia. Repeated blood transfusions cause an iron overload, triggering an enhanced generation of free radicals. In the present study, the anti-oxidant property of ethanolic extract of the leaves of Piper betle Linn. (PB) was evaluated in the erythrocytes from patients with HbE-beta thalassemia. In patients with HbE-beta thalassemia (n = 30) and age- and sex-matched healthy individuals (n = 30), the baseline level of reactive oxygen species (ROS) and free radical scavenging activity in the erythrocytes was measured by flow cytometry using dihydrodichlorofluorescein diacetate (H2DCFDA), in terms of the geometric mean fluorescence channel (GMFC). The baseline generation of ROS was significantly higher in the erythrocytes from patients with HbE-beta thalassemia, as compared to healthy volunteers, the GMFC being 67.20 ± 4.64 vs. 23.03 ± 1.88 (p<0.001), which was effectively decreased by PB. Similarly, H2O2 (0.5-1.0 mM) induced a higher increase in the GMFC in the erythrocytes from patients with HbE-beta thalassemia, as compared to controls which was effectively reduced by PB. Taken together, PB showed promising anti-oxidant activity against the erythrocytes from patients with HbE-beta thalassemia.
Page(s): 241-246
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.46(3) [June 2009]

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