NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(3) [June 2009] >


Title: Endopeptidases of Bacillus subtilis IBTC-3 and B. alcalophilus PB92 in synthesis of precursors of biologically active peptides
Authors: Głowacka, Agnieszka E
Szczesna-Antczak, Mirosława H
Piotrowicz-Wasiak, Małgorzata
Antczak, Tadeusz Z
Keywords: Subtilisin
Alkaline peptidase PB92
Immobilization
Amino acid esters
Peptides
Non-aqueous enzymology
Issue Date: Jun-2009
Publisher: CSIR
Abstract: Two endopeptidases (from Bacillus subtilis IBTC-3 and from B. alcalophilus PB92-commercial preparation) efficiently synthesized amino acid esters (NAc-Tyr-OEt and NAc-Phe-OEt) and dipeptides (NAc-Tyr-Gly-NH2 and NAc-Tyr-Arg-NH2) in organic solvent/water systems. The rate of NAc-Tyr-OEt synthesis mediated by the native subtilisin IBTC-3 was maximum (0.23 Umg-1) in ethanol/5-7% w/v water system, while the highest activity of the freeze-dried enzyme (0.18 Umg-1) was achieved, when water content was 9-10% w/v. The preferred system for dipeptide synthesis (using NAc-Tyr-OEt as acyl donor) by both the enzymes was acetonitrile/4% w/v water. In this system, the maximum yield of NAc-Tyr-GlyNH2 was 71 and 80% and that of NAc-Tyr-Arg-NH2 was 53 and 40% for subtilisin IBTC-3 and peptidase PB92, respectively. In contrast to the peptidase PB92, the subtilisin efficiently catalyzed esterification of NAc-Tyr with 1-butanol and isopropanol.
Page(s): 213-220
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.46(3) [June 2009]

Files in This Item:

File Description SizeFormat
IJBB 46(3) 213-220.pdf391.87 kBAdobe PDFView/Open
 Current Page Visits: 654 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 640159 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: nopr@niscair.res.in