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Title: Effective culture medium and combination of phytohormones for in vitro nodal culture of Shorea robusta Roxb. ex Gaertn
Authors: Kumar, Mrinal Nishant
Tirkey, Jyotshna
Rai, Suresh
Keywords: Activated charcoal;Anthereae mylitta;Breeding;Coconut water;Hardwood;Incubation;Sal;Shoot proliferation;Tasar silkworm;Timber
Issue Date: Dec-2018
Publisher: NISCAIR-CSIR, India
Abstract: Shorea robusta Roxb. ex Gaertn, commonly called Sal, is a major source of hardwood timber widely spread in the Indian sub continent and contributes to the biodiversity of the northern India. These woody plants are difficult to regenerate under in vitro conditions and only few attempts have been successful so far. The present investigation focus on testing two basal media for shoot proliferation from nodal and axillary shoot buds explants of S. robusta, each supplemented with different concentrations and combination of phytohormones. Shoot regenerations and percent of explants response, showed significant difference with media composition than with the prolonged incubation period during culturing. The result showed that with increased concentrations of benzyl amino purine (BAP) from 2.0 mg/L to 3.0 mg/L in the media WPM (McCown’s woody plant medium) and MMS (modified Murashige and Skoog medium), the number of shoot proliferations increases and alongwith significant difference in the percentage (80-83 %) of shoot initiations. To all the treatments (T1-T5), media supplemented with 1-naphthaleneacetic acid (NAA), gibberellic acid (GA3), and 6-aminopurine hemisulfate salt (AdSO4) were kept the same. However, in comparison with control (Tc) the rooting of in vitro cultured shoots in the half strength MMS medium containing indole-3-butyric acid (IBA) concentration between 1.5 and 2.5 mg/L with activated charcoal (AC) showed a significant difference. The above research finding has shown a potential to resolve some of the challenges associated with multiplication of important Sal cultivars and further improve the breeding methodologies through in vitro selection procedure.
Page(s): 914-921
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.56(12) [December 2018]

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