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Title: Induction of bioactive compounds in a co-cultured strain of Micromonosperma echinospora (DST-4) isolated from secluded Hirakud dyke soil
Authors: Pattnaik, Smaranika
Keywords: Micromonospora echinospora (DST-4) 16S rRNA gene;Coculture;Bioactive compound
Issue Date: Oct-2018
Publisher: NISCAIR-CSIR, India
Abstract: An actinomycetes (DST-4) strain was isolated from soil of Hirakud dyke, Western Odisha, India. The isolated actinomycetes strain was subjected to 16S rRNA gene partial sequencing and the sequence in FASTA format was analysed at National Centre Biotechnology Information (NCBI) portal using basic local alignment search tool (BLAST) analysis. The sequence was identified as a strain of gentamicin producing Micromonosperma echinospora with 99% identify. The sequence was submitted to GenBank and obtained an Accession no. MF536417.1. A phylogenetic tree was generated with six strains of same species available in database. The actinomycetes strain (DST-4) was screened for its antagonistic activity against three streptomycin resistant Staphylococcal aureus strains. ‘Triplicate patch inoculums diffusion’ and ‘cell immersed tube dilution’ techniques were used. Streptomycin was taken as reference drug. A quantitative cocultured-incubation method was applied for induction of bioactive compounds from the isolated actinomycete strain. The growth curve analysis of test staphylococcal strains through spectrophotometric as well as subculture experiments had inferred about antibacterial activity of putative bioactive compounds present in cell free extract (CFE) of DST-4 strain. The H1 NMR study of high performance liquid chromatography (HPLC) chromatogram peak fraction of CFE, was carried out. The nuclear magnetic resonance (NMR) signals had inferred about induction bioactive compounds with of R2NH and ROH as functional groups.
Page(s): 586-594
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(4) [October 2018]

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