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Title: HPAEC and MALDI-TOF-MS analysis of oligosaccharides generated from sesame meal by enzymatic hydrolysis
Authors: Chattopadhyay, Kaushik
Ghosh, Partha
Ghosal, Pradyot
Ray, Bimalendu
Keywords: Sesame meal;Enzyme hydrolysis;Oligosaccharides;GC;GC-MS;MALDI-TOF-MS;HPAEC
Issue Date: Mar-2007
Publisher: CSIR
IPC Code: Int.Cl.⁸ C07C
Abstract: Polysaccharides obtained from depectinated sesame meal by extraction with 1M KOH (1OH) have been fractionated by anion exchange chromatography yielding a neutral xyloglucan rich population (1OHaq). Size exclusion chromatography of 1OHaq has resulted in one peak between Kav 0.3 to 0.6, corresponding to an apparent molecular weight of 85 ± 25 kDa. The structure of xyloglucan has been investigated by enzyme degradation with endo-(1→4)-β-D-cellulase followed by analysis of the resulting fragments by chemical methods as well as high performance anion exchange chromatography (HPAEC) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). The result shows that the xyloglucan present in 1OHaq fraction contains hexa(1)-, hepta(2)-, octa(3)-, nona(4,5)- and deca(6)-saccharides as the building sub-units. Hydrolysis of 4M KOH extracted material (4OH) with endo-β-(1→4)-D-xylanase and analysis of the derived oligosaccharides shows that sesame meal xylan is composed of a β-(1→4)-linked-D-xylopyranose backbone substituted with 4-O-methyl-D-glucuronic acid residue.
Page(s): 471-477
ISSN: 0376-4699
Appears in Collections:IJC-B Vol.46B(03) [March 2007]

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