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Title: In vitro competence of vitrified bovine oocytes with open pulled straw
Authors: Dutta, D J
Sarmah, B C
Dev, Hiramoni
Raj, Himangshu
Keywords: Oocyte;Vitrification;in vitro maturation;in vitro fertilization
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: In vitro developmental competences of post thaw vitrified bovine cumulus oocyte complexes (COCs) were studied. Good qualities COCs were cryopreserved using vitrification solution comprising of 15 or 20% ethylene glycol (EG) + 15% or 20% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in tissue culture medium 199 (TCM199) with 10% fetal bovine serum (FBS). Immediately they were plunged in LN2 for ultra- rapid freezing using open pulled straw. Thawing and dilution were made stepwise. Post thaw normal vitrified and non vitrified oocytes were subjected to in vitro maturation and fertilization. The performance in respect of post thaw survivality and in vitro maturation on the basis of cumulus cell expansion was more than 80%. In comparison, the non-vitrified COCs group, in vitro maturation performance was 93.12%. The in vitro fertilization performances of vitrified immature and mature post thaw oocytes were recorded as 50.65% and 56.16% with 17.95% and 19.51% of blastocysts formation, respectively. Ultra rapid freezing protocol using vitrification with open pulled straw technique i.e. 7.5 or 10% EG + 7.5 or 10% DMSO for equilibration at room temperature for 3 min and 15 or 20% EG + 15 or 20% DMSO + 0.6M sucrose as vitrification solution within 1 min, on immature bovine oocyte yielded acceptable in vitro oocyte growth and embryonic development.
Page(s): 402-406
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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