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Title: Application of mobilization gene promoter for heterologous expression and curing of plasmid pSMA23
Authors: Sudhamani, M
Batish, V K
Heller, K J
Keywords: Lactobacillus;Promoter;Mobilization;Plasmid curing;Incompatibility
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: Lactobacilli have been recognized as key members of the group of probiotic bacteria by virtue of expressing a wide spectrum of physiological functions beneficial to human health. Genetic modification of this genus requires the availability of tools for gene expression like promoter and suitable host vector systems. In the context, plasmid pSMA23 was cured from its native host Lactobacillus casei A23 by electroporation with a construct based on the pSMA23 replicon. Cured strain A23 can be employed as host for vectors or constructs based on the replicon of pSMA23 and for heterologous expression of genes. The promoter less gene cat194 coding for chloramphenicol resistance was cloned under the control of the putative mobilisation gene promoter present on the native plasmid pSMA23 of Lactobacillus casei A23. Constructs were electroporated into Lactobacillus casei LK1 and transformants were obtained on media containing chloramphenicol, indicating expression of the cat194 gene. The putative promoter is thus active and can be recruited for gene expression.
Page(s): 416-421
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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