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Title: Cloning, sequencing and in silico analysis of β-glucosidase from Bacillus subtilis strain PS
Authors: Singh, Gopal
Kumar, Vinod
Dubey, Ashutosh
Agrawal, Sanjeev
Verma, A K
Keywords: β-Glucosidase;Glycosyl hydrolase family 1;Cloning;β-glucosidase;In silico analysis
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: Glycosyl hydrolases are group of enzymes classified into 132 known families based on sequence similarity. Glycosyl hydrolase family 1 comprises of number of enzymes with a known activities including β-glucosidases, which have wide diversity and known to have important applications. In the present study, the β-glucosidase gene of glycosyl hydrolase family 1 from Bacillus subtilis strain PS was successfully cloned and expressed in E. coli BL21 (DE3) by using pET28 (a) vector system. Sequence characterization revealed a 1407 bp long nucleotide sequence with an open reading frame (ORF) encoding 469 amino acids and a calculated molecular mass of 51.6 kDa. Its translated protein sequence was used for comparative analysis to reference protein sequences of β-glucosidases from different organisms for multiple sequence alignment, phylogenetic tree construction, variation in biochemical features and distribution of motifs using various bioinformatics tools. The analysis revealed important sequence features and properties of this important family which would be helpful in further classification, diversity study and genetic engineering of glycosyl hydrolase family 1 β-glucosidases.
Page(s): 431-440
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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